VEGF Expression in Umbilical Cord MSC Depends on the Patient’s Health, the Week of Pregnancy in Which the Delivery Took Place, and the Body Weight of the Newborn – Preliminary Report

Figures & data

Table 1 The Characteristics of the Study Group

Parameter	N	Mean	Median	Min	Max	SD	SE
Mother’a age	40	33.37	33.00	21.00	46.00	5.34	0.83
Pregnancies		2.10	2.00	1.00	5.00	1.07	0.17
Deliveries		1.80	2.00	1.00	5.00	0.93	0.14
HBD		38.17	38.00	34.00	40.00	1.34	0.21
Newborn weight [g]		3201.95	3190.00	2460.00	4060.00	392.97	61.37

Figure 1 Mesenchymal stem cells from a 4-day culture bright field microscopy (BF), 200× magnification, using Xcellence RT system with an IX81 inverted microscope (Olympus).

Figure 2 Cytometric evaluation of the presence of CD146, CD195, CD90, CD73 surface antigens on the analyzed MSCs. Navios Cytometer (Beckman Coulter).

Figure 3 Cytometric evaluation of cell viability using propidium iodide and annexin V. Navios cytometer (Beckman Coulter).

Figure 4 Cytometric evaluation of cell proliferation using Ki67 in analyzed MSCs. Navios Cytometer (Beckman Coulter).

Figure 5 Cytometric evaluation of the cell cycle phases of the analyzed MSCs. Navios Cytometer (Beckman Coulter).

Table 2 Relationships Between the Expression of the Studied Genes in MSC and the Age of the Mother, the Number of Pregnancies and Deliveries, the Gestation Week in Which the Delivery Oc-Curred and the Body Weight of the Newborn

Parameter	RQ VEGFA	RQ VEGFB	RQ VEGFC
RQ VEGFA	1.000	0.523*	0.394*
RQ VEGFB	0.523*	1.000	−0.131
RQ VEGFC	0.394*	−0.131	1.000
Mother’s age	−0.029	−0.170	0.226
Number of pregnancies	−0.148	−0.188	−0.209
The number of deliveries	−0.106	−0.180	−0.130
HBD	0.113	−0.345*	0.111
Newborn weight	−0.328*	−0.186	0.125

Note: *p < 0.05 Spearman correlations.

Figure 6 Graph of distribution newborn weight and VEGFA gene expression. *p < 0.05 Spearman correlations.

Figure 7 Mean (RQ±SE) expression VEGFB gene in MSC, depending on the time of childbirth, *p < 0.05 U Mann Whitney Test.

Figure 8 Graph of distribution HBD and VEGFB gene expression. *p < 0.05 Spearman correlations.

Figure 9 Mean (RQ±SE) expression of VEGFA and VEGFC genes in MSC, depending on the prevalence of hypertension in pregnant women, *p < 0.05 U Mann Whitney Test.

Figure 10 Mean (RQ±SE) expression VEGFA gene in MSC, depending on prevalence of hyperthyroidism, *p < 0.05 U Mann Whitney Test.