Figures & data
Figure 1 Isolation process and potential therapeutic products derived from lipoaspirate.
Notes: Lipoaspirate is harvested from a patient. Digestion with collagenase produces a stromal vascular fraction (SVF), a combination of immune cells, adipose-derived stem cells (ASC), endothelial progenitor cells, and others. ASC can be purified from SVF by culturing adherent proliferative cells and removing nonadherent cells. SVF and ASC are commonly used as autologous therapeutics and can be preserved for future use. Both SVF and ASC have the potential to be used as allogeneic therapeutics. Differentiated ASC and the factors secreted by ASC also have potential therapeutic use.
Figure 2 Fluorescence-activated cell sorting characterization of (A) nonhematopoietic (CD45-) cells of stromal vascular fraction (SVF) and (B) adherent purified adipose-derived stem cells (ASC). (C) Summary of flow cytometry cell surface marker expression analysis for uncultured endothelial progenitor cells (EPC) in SVF, uncultured ASC in SVF, and adherent purified ASC.
Notes: SVF contains two main nonhematopoietic (CD45−) cell populations, ASC and EPC. When ASC are purified from SVF by adherent culture, CD34 expression is lost and CD105 expression is increased. Adapted from Feisst V, Brooks AE, Chen CJ, Dunbar PR. Characterization of mesenchymal progenitor cell populations directly derived from human dermis. Stem Cells Dev. 2014;23(6):631–642.Citation3 Copyright © 2014.
Figure 3 Potential mechanisms of effect of adipose-derived stem cells (ASC).
Figure 4 Clinical trials registered on http://www.ClinicalTrials.gov focused on adipose stem or stromal cells as at April 2015.
Notes: Only clinical trials which were currently open were included. Allocation to the single most appropriate “condition targeted” was made by the senior author from the clinical trial title and condition(s) listed. Each trial was therefore allocated to a single condition only.
