The Influence of IL-1B Gene Polymorphisms on H. pylori Infection and Triple Treatment Response Among Jordanian Population

Figures & data

Table 1 PCR and Restriction Conditions of IL-1B Gene Polymorphisms

Polymorphism	Primers: 5ʹ-3ʹ	PCR Conditions	Restriction Conditions	Fragment Sizes
T-31C	F: AGA AGC TTC CAC CAA TAC TC R: ACC ACC TAG TTG TAA GGA	35 cycles: 94°C for 30s, 61°C for 45s, 72°C for 45s	Alu I, 37°C, 16 h	C allele: 239 T allele: 152, 87
C+3954T	F: GTT GTC ATC AGA CTT TGA CC R: TTC AGT TCA TAT GGA CCA GA	35 cycles: 94°C for 45s, 55°C for 45s, 72°C for 45s	TaqI, 37°C, 16 h	T allele: 249 C allele:135, 114

Table 2 Demographics of Participants

Parameter	Control Group	Cases Group
Mean age ± SD	42.6 ± 14.7	40.8 ± 15.2
Gender (%)
Male	41.3%	49%
Female	48.7%	51%
Mean BMI ± SD	28.0 ± 4.51	28.49 ± 5.22
Hypertension (%)	16.1%	22.2%
Diabetes mellitus	9.0%	13.6%
Thyroid dysfunction	5.1%	3.5%
Kidney/liver diseases	1.9%	3.1%

Abbreviations: SD, standard deviation; BMI, body mass index.

Table 3 Association Between IL1B SNPs and H. pylori Infection

IL-1β SNP	Cases	Controls	Odds Ratio (95% CI)	P value
C3954T	N (%)	N (%)
CC	117 (46)	55 (35)	1.00
CT	123 (48)	90 (58)	1.56 (1.02–2.37)	0.12
TT	17 (7)	10 (6)	1.25 (0.54–2.91)
Allele C	357 (69)	200 (65)
Allele T	157 (31)	110 (35)		0.14
T31C	N (%)	N (%)
CC	46 (17.9)	30 (19.4)	1.00
TC	82 (31.9)	65 (41.9)	1.70 (1.09–2.66)	0.043
TT	129 (50.2)	60 (38.7)	1.40 (0.81–2.44)
Allele C	174 (34)	125 (40)
Allele T	340 (66)	185 (60)		0.061

Abbreviation: CI, confidence interval.

Table 4 Analysis of IL-1B Haplotypes of the Examined Polymorphisms

	Cases Frequency	Controls Frequency	Odds Ratio (95% CI)	P-value
CT	38.2	32.82	1.00	——
CC	31.27	31.7	1.12 [0.78–1.60]	0.55
TT	27.97	26.86	1.19 [0.77–1.83]	0.44
TC	2.58	8.62	4.04 [1.77–9.20]	0.001

Abbreviation: CI, confidence interval.

Table 5 IL-1B SNPs Association with Triple Therapy Response

IL-1β SNP	Resistance to Triple Therapy	Responsive to Triple Therapy	Odds Ratio (95% CI)	P-value
CC	19 (46)	98 (46)	1.00
CT	19 (46)	104 (48)	1.06 (0.53–2.12)	0.97
TT	3 (7)	14 (6)	0.90 (0.24–3.46)
T31C	N (%)	N (%)
CC	6 (15)	40 (19)	1.00
TC	10 (24)	72 (33)	2.00 (0.88–4.52)	0.19
TT	25 (61)	104 (48)	1.67 (0.64–4.35)