Osteopontin Gene Polymorphisms rs1126616 C>T and rs1126772 A>G are Associated with Atopic Dermatitis in Polish Population

Figures & data

Table 1 Characteristics of AD Patients and Controls. Data are Expressed as Minimum – Maximum, Means ± SD or n (%)

	AD	Controls
Subjects	45	137
Sex, n (%)
● Female	28 (62.2)	85 (62)
● Male	17 (37.8)	52 (38)
Age (years), min – max (mean ± SD)	14–66 (30.62 ± 10.58)	18–56 (34.19 ± 8.20)
Years of disease, min – max (mean ± SD)	1–53 (22.27 ± 9.14)
History of asthma, n (%)	14 (31.1)
History of allergic rhinitis, n (%)	24 (53.3)
History of nettle rash, n (%)	15 (33.3)
History of conjunctivitis, n (%)	22 (48.9)
History of impetiginisation, n (%)	28 (62.2)
Severity (SCORAD index):
● Moderate (25–50 points)	6 (13.3)
● Severe (> 50 points)	39 (86.7.1)

Abbreviations: AD, atopic dermatitis; SCORAD, scoring atopic dermatitis; SD, standard deviation.

Table 2 Genotypes and Alleles Frequency of OPN rs1126616 C>T, rs1126772 A>G, rs9138 A>C, and rs3841116 T>G Polymorphisms in AD Patients and Controls. Data are Expressed as n (%)

rs1126616 C>T
	Genotypes			Alleles
	CC	CT	TT	C	T
AD patients, n (%)	23 (51.1)	15 (33.3)	7 (15.6)	61 (67.8)	29 (32.2)
Control, n (%)	85 (62)	47 (34.3)	5 (3.7)	216 (78.8)	58 (21.2)
Statistics	χ^2 = 7.97, df = 2, P = 0.019 OR = 4.86, 95% CI = 1.46–16.20			χ^2 = 3.96, df = 1, P = 0.047 OR = 1.77, 95% CI = 1.04–3.00
rs1126772 A>G
	Genotypes			Alleles
	AA	AG	GG	A	G
AD patients, n (%)	19 (42.2)	16 (35.6)	10 (22.2)	54 (60)	36 (40)
Control, n (%)	78 (56.9)	48 (35.1)	11 (8)	204 (74.5)	70 (25.5)
Association	χ^2 = 7.29, df = 2, P = 0.026 OR = 3.27, 95% CI = 1.29–8.33			χ^2 = 6.17, df = 1, P = 0.013 OR = 1.94, 95% CI = 1.18–3.21
rs9138 A>C
	Genotypes			Alleles
	AA	AC	CC	A	C
AD patients, n (%)	24 (53.3)	16 (35.6)	5 (11.1)	64 (71.1)	26 (28.9)
Control, n (%)	75 (54.7)	48 (35.1)	14 (10.2)	198 (72.3)	76 (27.7)
Association	χ^2 = 0.04, df = 2, P = 0.980 OR = 1.10, 95% CI = 0.37–3.24			χ^2 = 0.01, df = 1, P = 0.920 OR = 1.06, 95% CI = 0.62–1.79
rs3841116 T>G
	Genotypes			Alleles
	TT	TG	GG	T	G
AD patients, n (%)	44 (97.8)	1 (2.2)	0 (0)	89 (98.9)	1 (1.1)
Control, n (%)	136 (99.3)	1 (0.7)	0 (0)	273 (99.6)	1 (0.4)
Association	χ^2 = 0.07, df = 2, P = 0.708 OR = 3.09, 95% CI = 1.90–50.46			χ^2 = 0.00, df = 1, P = 1.000 OR = 3.04, 95% CI = 1.19–49.20

Notes: For each polymorphism ORs with 95% CI were calculated under recessive and allele-frequency genetic models.

Abbreviations: AD, atopic dermatitis; OR, odds ratio; CI, 95% confidence interval for the odds ratio.

Table 3 Association Between Clinical Manifestations of AD and OPN rs1126616 C>T, rs1126772 A>G, rs9138 A>C, and rs3841116 T>G Genotypes/Alleles. Data are Expressed as n or n (%)

Manifestation	rs1126616 C>T Genotypes/Alleles Distribution					P value, OR (95% CI)
	CC n = 23	CT n = 15	TT n = 7	C n = 61	T n = 29
Asthma	3 (13)	7 (46.7)	4 (57.1)	13 (21.3)	15 (51.7)	P^a = 0.017, 3.73 (0.71–19.67) P^b = 0.004, 3.96 (1.53–10.25)
Allergic rhinitis	11 (47.8)	9 (60)	4 (57.1)	31 (50.8)	17 (58.6)	P^a = 0.782, 1.2 (0.24–6.11) P^b = 0.639, 1.37 (0.56–3.35)
Nettle rash	6 (26.1)	6 (40)	3 (42.9)	18 (29.5)	12 (41.4)	P^a = 0.625, 1.63 (0.31–8.43) P^b = 0.380, 1.68 (0.67–4.24)
Conjunctivitis	12 (52.2)	5 (33.3)	5 (71.4)	29 (47.5)	15 (51.7)	P^a = 0.219, 3.09 (0.53–17.95) P^b = 0.888, 1.18 (0.49–2.86)
Impetiginisation	12 (52.2)	11 (73.3)	5 (71.4)	35 (57.4)	21 (72.4)	P^a = 0.383, 1.63 (0.28–9.52) P^b = 0.252, 1.95 (0.75–5.09)
Manifestation	rs1126672 A>G genotypes/alleles distribution					P value, OR (95% CI)
	AA n = 19	AG n = 16	GG n = 10	A n = 54	G n = 36
Asthma	2 (10.5)	6 (37.5)	6 (60)	10 (18.5)	18 (50)	P^a = 0.016, 5.06 (1.14–22.49) P^b = 0.002, 4.40 (1.71–11.35)
Allergic rhinitis	9 (47.4)	7 (43.8)	8 (80)	25 (46.3)	23 (63.9)	P^a = 0.156, 4.75 (0.88–25.61) P^b = 0.154, 2.05 (0.86–4.88)
Nettle rash	6 (31.6)	5 (31.3)	4 (40)	17 (31.5)	13 (36.1)	P^a = 0.878, 1.45 (0.34–6.22) P^b = 0.093, 1.23 (0.51–3.00)
Conjunctivitis	9 (47.4)	9 (56.3)	4 (40)	27 (50)	15 (41.7)	P^a = 0.712, 0.63 (0.15–2.63) P^b = 0.578, 0.71 (0.31–1.67)
Impetiginisation	12 (63.2)	9 (56.3)	7 (70)	33 (61.1)	23 (63.9)	P^a = 0.775, 1.89 (0.42–8.43) P^b = 0.791, 1.13 (0.47–2.70)
Manifestation	rs9138 A>C genotypes/alleles distribution					P value, OR (95% CI)
	AA n = 24	AC n = 16	CC n = 5	A n = 64	C n = 26
Asthma	6 (25)	6 (37.5)	2 (40)	18 (28.1)	10 (38.5)	P^a = 0.634, 1.56 (0.23–10.53) P^b = 0.480, 1.59 (0.61–4.17)
Allergic rhinitis	11 (45.8)	9 (56.3)	4 (80)	31 (48.4)	17 (65.4)	P^a = 0.362, 4.00 (0.41–39.00) P^b = 0.219, 2.01 (0.78–5.17)
Nettle rash	7 (29.2)	6 (37.5)	2 (40)	20 (31.3)	10 (38.5)	P^a = 0.815, 1.38 (0.21–9.33) P^b = 0.680, 1.38 (0.53–3.56)
Conjunctivitis	14 (58.3)	7 (43.8)	1 (20)	35 (54.7)	9 (34.6)	P^a = 0.259, 0.23 (0.02–2.21) P^b = 0.135, 0.44 (0.17–1.13)
Impetiginisation	17 (70.8)	9 (56.3)	2 (40)	43 (67.2)	13 (50)	P^a = 0.359, 0.36 (0.05–2.41) P^b = 0.199, 0.49 (0.19–1.24)
Manifestation	rs3841116 T>G genotypes/alleles distribution					P value
	TT n = 44	TG n = 1	GG n = 0	T n = 89	G n = 1
Asthma	13 (29.5)	1 (100)	0 (0)	27 (30.3)	1 (100)	P^a = 0.311 P^b = 0.311
Allergic rhinitis	24 (54.5)	0 (0)	0 (0)	24 (27)	0 (0)	P^a = 0.467 P^b = 1.00
Nettle rash	14 (31.8)	1 (100)	0 (0)	29 (32.6)	1 (100)	P^a = 0.333 P^b = 0.333
Conjunctivitis	22 (50)	0 (0)	0 (0)	44 (49.4)	0 (0)	P^a = 1.00 P^b = 1.00
Impetiginisation	27 (61.4)	1 (100)	0 (0)	55 (61.8)	1 (100)	P^a = 1.00 P^b = 1.00

Notes: P^a, comparison of genotypes between AD patients with and without a particular manifestation performed by Fisher test; P^b, comparison of alleles between AD patients with and without a particular manifestation performed by Fisher test. For each polymorphism ORs with 95% CI were calculated under recessive and allele-frequency genetic models.

Abbreviations: AD, atopic dermatitis; OR, odds ratio; 95% CI, 95% confidence interval of the odds ratio.