Comparison of chemiluminescence immunoassay, enzyme-linked immunosorbent assay and passive agglutination for diagnosis of Mycoplasma pneumoniae infection

Figures & data

Figure 1 Antibody type and total antibody measured by CLIA (A) and ELISA (B) in PA-positive group.

Note: There was a decreasing trend of MP-IgM negative ratio while there was an increasing trend of MP-lgG positive ratio measured by CLIA and ELISA in PA-positive group (titer ≥1:80).
Abbreviations: CLIA, chemiluminescence immunoassay; ELISA, enzyme linked immunosorbent assay; Ig, immunoglobulin; MP, Mycoplasma pneumoniae; PA, passive agglutination.

Table 1 MP antibody detected by CLIA, ELISA, and PA

PA	CLIA						ELISA						Total
	MP-IgM		MP-IgG		MP-IgG+MP-IgM		MP-IgM		MP-IgG		MP-IgG+MP-IgM
	Negative	Positive	Negative	Positive	Negative	Positive	Negative	Positive	Negative	Positive	Negative	Positive
<1:40	44	13 (22.8%)	57	0	44	13	38	19 (33.3%)	54	3	37	20	57
1:40	21	22 (51.2%)	42	1	20	23	20	23 (53.5%)	39	4	20	23	43
1:80	12 (40%)	18	26	4 (13.3%)	11 (36.7%)	19	13 (43.3%)	17	26	4 (13.3%)	12 (40%)	18	30
1:160	9 (18%)	41	37	13 (26%)	7 (14%)	43	4 (8%)	46	29	21 (42%)	2 (4%)	48	50
1:320	5 (14.3%)	30	20	15 (42.9%)	3 (8.6%)	32	5 (14.3%)	30	15	20 (57.1%)	2 (5.7%)	33	35
1:640	2 (10%)	18	5	15 (75%)	0	20	1 (5%)	19	1	19 (95%)	0	20	20
≥1:1,280	3 (6.7%)	42	4	41 (91.1%)	0	45	3 (6.7%)	42	2	43 (95.6%)	0	45	45
Total	96	184	191	89	85	195	84	196	166	114	73	207	280

Note: Some data includes percentages to make the analysis of the experimental results clearer.

Abbreviations: CLIA, chemiluminescence immunoassay; ELISA, enzyme-linked immunosorbent assay; Ig, immunoglobulin; MP, Mycoplasma pneumoniae; PA, passive agglutination.

Figure 2 The consistency analysis of CLIA and PA.

Note: In the PA-positive group, the consistent percentages of the two types of antibodies increased with raised MP antibody titers.
Abbreviations: CLIA, chemiluminescence immunoassay; Ig, immunoglobulin; MP, Mycoplasma pneumoniae; PA, passive agglutination.

Figure 3 The consistency analysis of ELISA and PA.

Notes: In the PA-positive group, the consistent percentages of the two types of antibodies increased with raised MP antibody titers.
Abbreviations: ELISA, enzyme-linked immunosorbent assay; Ig, immunoglobulin; MP, Mycoplasma pneumoniae; PA, passive agglutination.

Table 2 The consistency of CLIA and ELISA with PA

Consistency coefficient %	PA							Total
	<1:40	1:40	1:80	1:160	1:320	1:640	≥1:1,280
CLIA
MP-IgM	77%	49%	60%	82%	86%	90%	93%	76%
MP-IgG	100%	98%	13%	26%	43%	75%	91%	67%
MP-IgG+IgM	77%	47%	62%	86%	91%	100%	100%	80%
ELISA
MP-IgM	67%	47%	57%	92%	86%	95%	93%	76%
MP-IgG	95%	91%	13%	42%	57%	95%	96%	71%
MP-IgG+IgM	65%	47%	60%	96%	94%	100%	100%	79%

Abbreviations: CLIA, chemiluminescence immunoassay; ELISA, enzyme-linked immunosorbent assay; Ig, immunoglobulin; MP, Mycoplasma pneumoniae; PA, passive agglutination.

Figure 4 The consistency analysis of CLIA and ELISA in typing MP antibody.

Note: The results showed consistency between CLIA and ELISA.
Abbreviations: CLIA, chemiluminescence immunoassay; ELISA, enzyme-linked immunosorbent assay; Ig, immunoglobulin; MP, Mycoplasma pneumoniae.

Table 3 The consistency of CLIA and ELISA in MP antibody typing

	MP-IgM	MP-IgG	MP-IgG+IgM
Consistency coefficient (%)	91.4	88.9	92.1
Negative consistency (%)	92.9	98.2	93.2
Positive consistency (%)	90.8	75.4	91.8
Kappa	0.804	0.763	0.806

Abbreviations: CLIA, chemiluminescence immunoassay; ELISA, enzyme-linked immunosorbent assay; Ig, immunoglobulin; MP, Mycoplasma pneumoniae.