Polysaccharide peptide (PsP) Ganoderma lucidum: a potential inducer for vascular repair in type 2 diabetes mellitus model

Figures & data

Table 1 Result in each group with one-way ANOVA and posthoc duncan test

Parameter	Treatment variant groups					P-value (P<0.05)
	Normal	Control (+)	PsP 50 mg/kgBW	PsP 150 mg/kgBW	PsP 300 mg/kgBW
H2O2 (Nmol)	14,25±3,30	84,28±35,20	64,67±40,61	40,93±23,06	28,47±8,01	0,003*
	(9,25–18,00)	(62,13–145,50)	(10,38–171,63)	(26,50–80,13)	(21,75–41,88)
EPC (Sel/mL)	664,40±427,98	298,60±166,31	412,60±74,51	585,00±30,24	941,00±227,84	0,000*
	(522–1231)	(285–451)	(296–489)	(557–630)	(554–1132)
CEC (Sel/mL)	82,80±21,03	678,20±47,48	306,40±100,98	203,00±50,43	133,60±12,95	0,000*
	(62–105)	(602–727)	(139–411)	(150–255)	(117–152)
Rasio EPC:CEC	8,25±5,98	0,44±0,26	1,62±1,09	3,01±0,65	6,97±1,26	0,001*

Notes: The results are presented in mean ± SD (range). All values of each parameter are already matriculated in the math international standard form (decimal).*P<0.05 is considered as significant.

Abbreviations: PSP, polysaccharide peptide; EPC, endothelialprogenitor cell; CEC, circulating endothelial cell.

Figure 1 The result of flow cytometry of EPC counted by CD 133(+)-dominant cells located at the lower right quadrant of the graph.

Notes: (A) Normal group, (B) insulin resistance models, (C) group with administration 50 mg/kgBW dose of PsP, (D) group with administration 150 mg/kgBW dose of PsP, (E) group with administration 300 mg/kgBW dose of PsP.
Abbreviations: EPC, endothelial progenitor cell; BW, body weight; PsP, polysaccharide peptide.

Figure 2 Single and double staining representative circulating cell target as well as vascular repair indicator.

Note: (A) Marker used for circulating endothelial progenitor cell identification, (B) marker used for circulating endothelial cell identification.
Abbreviation: CD, cluster of differentiation.

Figure 3 The result of flow cytometry of CEC counted by CD 146(+)/CD45(-) cells located at the upper left quadrant of the graph.

Notes: (A) Normal group, (B) insulin resistance models, (C) group with administration 50 mg/kgBW dose of PsP, (D) group with administration 150 mg/kgBW dose of PsP, (E) group with administration 300 mg/kgBW dose of PsP.
Abbreviations: CEC, circulating endothelial cell; BW, body weight; PsP, Pplysaccharide peptide.