Corneal endothelial cell density and morphology in Phramongkutklao Hospital

Figures & data

Table 1 Characteristics of the subjects

General data	No. (%)
Gender
Male	90(44.6)
Female	112(55.4)
Age (Year: mean ± SD)	45.73 ± 16.12
Underlying disease
Yes	48(23.8)
No	154(76.2)
Eye drop drug use
Yes	15(7.4)
No	187(92.6)
Smoking
Yes	38(81.8)
No	164(18.8)
Number of smoking (pack/day) (mean ± SD)	10.61 ± 7.87
Smoking duration (Year: mean ± SD)	15.53 ± 12.59
Now stop smoking
Yes	23(60.53)
No	15(39.47)
Duration of stop smoking (year)	10.57 ± 10.79
Visual acuity (uncorrected)
20/20 – 20/50	183(90.6)
20/70 – PL	19(9.4)
Mean intraocular pressure	13.76 ± 3.17 mmHg
Mean Schirmer’s test	8.09 ± 6.13 mm

Table 2 Endothelial cell characteristics of the study population in different gender

	Male	Female	p-valuea	Total
No. of eyes	180	224		404
Age (year)	41.73 ± 18.21	48.94 ± 13.46	0.002	45.73 ± 16.12
Cell density (cells/mm^2)	2679.98 ± 361.89	2578.10 ± 284.93	0.002	2623.49 ± 325.06
Cell size variability (%)	37.94 ± 7.13	40.62 ± 8.86	0.001	39.43 ± 8.23
Hexagonality	53.77 ± 10.69	49.68 ± 10.92	<0.001	51.50 ± 10.99

a Notes: Unpaired t-test.

Figure 1 Correlation between MCD and age.

Table 3 Endothelial cell characteristics of the study population in different age groups

Age group (years)	No. of eyes	Cell density (cells/mm^2)a	Cell size variability (%)a	Hexagonalitya
20–30	94	2799.02 ± 260.44	38.88 ± 6.88	54.41 ± 11.57
31–40	50	2744.60 ± 236.85	38.73 ± 8.52	50.86 ± 9.11
41–50	104	2642.66 ± 304.55	39.61 ± 7.97	50.02 ± 10.85
51–60	78	2553.12 ± 266.88	40.19 ± 7.26	49.84 ± 11.48
over 61 year	78	2379.00 ± 356.30	39.53 ± 10.59	52.04 ± 10.59

a Notes: Mean ± SD.

Table 4 Correlation between parameters and other factors

Factors	MCD	%CV	Hexagonality
Age	0.000	0.310	0.022
Gender	0.002	0.001	0.000
Smoking	0.759	0.972	0.875
Underlying disease	0.931	0.825	0.437
Long term eye drug use	0.213	0.227	0.507
IOP	0.881	0.510	0.825
Schirmer’s test	0.079	0.127	0.012

Abbreviations: MCD, mean endothelial cell density; CV, coefficient of variation; IOP, intraocular pressure.

Notes: p-value < 0.05.

Table 5 Multiple regression analysis between parameters and univariated significant factors

	B	E(B)	t	p-value	95% CI
MCD
constant	3051.939		50.689	<0.001	2933.209, 3170668
age	−8.959	−0.458	−7.125	<0.001	−11.438, −6.479
gender	−26.593	−0.042	−0.654	0.514	−106.796, 53.610
CV
constant	37.936		62.404	<0.001	36.741, 39.131
gender	2.687	0.162	3.295	0.001	1.084, 4.290
HEXA
constant	55.187		19.138	<0.001	49.501, 60.874
age	−0.085	0.050	−1.715	0.088	−0.184, 0.013
gender	−2.370	−0.106	−1.439	0.152	−5.619, 0.878
Schirmer’s test	0.152	0.076	1.047	0.296	−0.134, 0.439

Table 6 Comparison of endothelial cell density

Age group (Years)	Phramongkutklao Hospital		India		Japan		America
	No. of eyes	MCD (cell/mm^2)a	No. of eyes	MCD (cell/mm^2)a	No. of eyes	MCD (cell/mm^2)a	No. of eyes	MCD (cell/mm^2)a
21–30	94	2799 ± 260	104	2782 ± 250	18	3893 ± 259	11	2977 ± 324
31–40	50	2744 ± 236	96	2634 ± 288	10	3688 ± 245	6	2739 ± 208
41–50	104	2642 ± 304	97	2408 ± 274	10	3749 ± 407	11	2619 ± 321
51–60	78	2553 ± 266	98	2438 ± 309	10	3386 ± 455	13	2625 ± 172
61–70	50	2485 ± 267	88	2431 ± 357	6	3307 ± 330	8	2684 ± 384
>70	28	2189 ± 416	54	2360 ± 357	15	3289 ± 313	15	2431 ± 339

Abbreviations: MCD, mean endothelial cell density; SD, standard deviation.

a Notes: Mean ± SD

b Rao et al 2000

c Matsuda et al 1985.

RaoSKRanjanSPFoglaR2000Cornea endothelial cell density and morphology in normal Indian eyesCornea19820311095057

 PubMed Web of Science ®Google Scholar

MatsudaMYeeRWEdelhauserHF1985Comparison of the corneal endothelium in an American and a Japanese populationArch Ophthalmol10368703977679

 PubMedGoogle Scholar