Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis

Figures & data

Figure 1 Location of 13 microsatellite markers used in this study. The heavy bar above the chromosome represents the 2p22.1-p22.3 region including the GLC3A locus.

Table 1 Primer sequences of 13 microsatellite markers used in this study

Locus	Dye label		Primer sequences (5′-to-3′)	Allele size range
D2S2230	VIC	F	CTTAGAAACGAACCACTGGA	224–232
		R	GTGGAAATAAGTGCAACCAT
D2S1895i	VIC	F	AAGGATAACCACTCTATCTGCTAC	285–295
		R	GAGCTCTGATTCTATGTATCAAAG
D2S0367i	6-FAM	F	CTATCAATTCCTGCATCTAACTAT	297–335
		R	TAGTTACTGGATGGCTGTGTAC
D2S1501i	PET	F	AGTCAATCTGGACTGCTGG	124–142
		R	AGCCCTCAGTACGCAGTAG
D2S1243i	NED	F	TCCTCAGATTCACTAAACTCATAC	284–298
		R	TGTCAAGTGATGTTAAGTGCTA
D2S177	PET	F	AGCTCAGAGACACCTCTCCA	287–311
		R	CTGTATTAGGATACTTGGCTATTGA
D2S0973i	6-FAM	F	AGTTGTAAAGTATCAAGCCATAAA	243–269
		R	GCAGAATGGAACTTAGTGTCTA
D2S2331	NED	F	ATTAGCACTTACCTGGCACA	126–140
		R	AGTTTATGCTGTGATTAATACCTGG
D2S0416i	6-FAM	F	CACTCAGTGGCCATTAGAG	440–454
		R	GTAGATTCAGAATTTATGAACCAC
D2S0425i	PET	F	CTTAAGTACCTTCATGAAGTTCAG	254–260
		R	CAGGAGTTCAAGGTTAAAGTAAG
D2S0161i	NED	F	GTCTAAGTCAGTAGCATAGCCAAG	292–322
		R	ACGGCTTGAAGTTATAGGAGAC
D2S0099i	PET	F	GCTAACTTTAACTGATTAAGCAAA	351–363
		R	CATTACCAGTGAGCCTCAC
D2S1903i	NED	F	TTACCAGTATGCCACTAAACTTAC	242–268
		R	CAGCTTTCTTAATACAGCAAAG

Table 2 Frequencies of 13 microsatellite markers in NTG cases and controls

			Frequency, n (%)						Odds ratio
Marker	No. of alleles	Allele*	Cases (2n = 284)		Controls (2n = 202)		P	Pc	(95% CI)
D2S2230	5	228	135	(47.5)	102	(50.5)	0.67
D2S1895i	6	285	19	(6.7)	15	(7.4)	0.75
D2S0367i	14	309	33	(11.6)	36	(17.8)	0.054
D2S1501i	12	136	18	(6.3)	15	(7.4)	0.64
D2S1243i	8	294	13	(4.6)	16	(7.9)	0.13
D2S177	13	297	52	(18.3)	48	(23.8)	0.14
D2S0973i	8	253	226	(79.6)	164	(81.2)	0.66
D2S2331	8	132	250	(88.0)	170	(84.2)	0.22
D2S0416i	5	444	44	(15.5)	48	(23.8)	0.022	0.11	0.59 (0.37–0.93)
D2S0425i	4	258	10	(3.5)	16	(7.9)	0.034	0.13	0.42 (0.19–0.96)
D2S0161i	7	297	14	(4.9)	16	(7.9)	0.18
D2S0099i	7	353	7	(2.5)	11	(5.4)	0.086
D2S1903i	12	262	12	(4.2)	5	(2.5)	0.30

Notes: *Alleles with >1% of frequency and found to have the most difference in allele frequency between cases and controls in each marker are shown. Each allele was named by the size of its amplification.