Association of microsatellite polymorphisms of the GPDS1 locus with normal tension glaucoma in the Japanese population

Figures & data

Figure 1 Location of 20 microsatellite markers used in this study and the genes around the GPDS1 locus. Display of genes and chromosome seven results in the UCSC genome browser on Human Mar. 2006 Assembly at chr7: 151,120,000–158,120,000.

Table 1 Primers used in polymerase chain reaction for amplification of microsatellite markers in this study

Location	Orientationa	Sequence
D7S0954i	F (NED)	5′TTACATAAGGTACTTTCTGGTCAT3′
	R	5′TGGTCTCCAGCTACATCAG3′
D7S0947i	F (NED)	5′CTACTTTCCTGGTGTCTAGCTT3′
	R	5′GATAACAGCTGATAGGTAGATCAG3′
D7S798	F (VIC)	5′AGCTGCAAAATAGTGGAAGTAG3′
	R	5′CATCAATTCACATAATGACCG3′
D7S1829	F (VIC)	5′GGGGGTTTCAGAAGAGATGT3′
	R	5′CCTCCAAAATTTCTGAGCAA3′
D7S2462	F (FAM)	5′GCCAAGATTGCACCACTAC3′
	R	5′TGTTAAAAGTTCCCCAAGC3′
D7S0585i	F (NED)	5′AATGAGCATTCTCAGTTTGAATAC3′
	R	5′ATCTGGTGAACGAGTAAATAACAC3′
D7S2546	F (FAM)	5′GGAGGTTGAACAACTCTGAATAC3′
	R	5′CACGCCAGGGTCTATCTT3′
D7S2447	F (NED)	5′GTCAGATGTAGGAACCAAGC3′
	R	5′TCTCACTGTACTCAGCCTGT3′
Z67544	F (VIC)	5′CAACTTAAAATACGCTGTTACC3′
	R	5′GCTTTTTCAGACCAAATAACTTAC3′
D7S1112i	F (NED)	5′GAGAATCTAATGAAAGCTGTGG3′
	R	5′TTCAGGGCATCCAAGAAG3′
D7S0952i	F (NED)	5′AGATATTTGGCTAAACATGGTC3′
	R	5′TCTTCAGAGATACAGAAGCAATAG3′
D7S550	F (FAM)	5′TCTCATCTGTGAATGCACTATC3′
	R	5′GCAGTTGGGTTATTTCAAGTC3′
D7S1272i	F (PET)	5′AATGTCCTGGAGGCTGAG3′
	R	5′AAAGACTTGGTGAGACCTTCTC3′
D7S2465	F (PET)	5′ACCTGGGCAACAGAGTGAG3′
	R	5′CTTCAAAGAGTTTATGCTTATGTGG3′
D7S1520i	F (PET)	5′GCTGTTAGCATCTGGTTAATTTAC3′
	R	5′AAACTGAAGTCTGCCATCTATTAG3′
D7S1108i	F (FAM)	5′ACTTACTTATCCTACTGATCCGTG3′
	R	5′TGAACCATAAATTACCTCCATTC3′
D7S1191i	F (VIC)	5′CACTCTATTTGCATGGTGAAC3′
	R	5′TCCAGTAGAGGGAGCTCAG3′
D7S0968i	F (NED)	5′CACATCCTTTATACCTACATTCAG3′
	R	5′TAATTTATCTAGAAGGTCAGCAAA3′
D7S2423	F (NED)	5′CTTCAGACCTTCAGTTGATGAT3′
	R	5′GCTCTCAGACACATTTTCCA3′
D7S1159i	F (VIC)	5′CTTGGAGTCAAAGAGGCC3′
	R	5′CTTTCCTGACTCCTTGATTAGAG3′

Note: ^aDye label.

Table 2 Frequencies of 20 microsatellite markers in normal-tension glaucoma (NTG) patients and healthy participants

Marker	Occurrence of allele	Allelea	Frequencies (%)		P	Pc	Odds ratio (95% CI)
			NTG patients (2n = 282)	Healthy participants (2n = 202)
D7S0954i	9	310	3.5	2.0	0.31
D7S0947i	7	151	3.2	2.5	0.64
D7S798	8	211	7.8	4.0	0.08
D7S1829	13	391	6.0	4.0	0.31
D7S2462	15	132	14.5	26.2	0.0013	0.019	0.48
D7S0585i	9	324	4.3	3.0	0.46
D7S2546	8	239	13.8	6.4	0.0096	0.077	2.33
D7S2447	4	175	53.5	52.5	0.82
Z67544	6	151	6.4	1.5	0.0091	0.055	3.99
D7S1112i	6	121	44.7	42.1	0.57
D7S0952i	3	450	1.2	0.5	0.21
D7S550	13	192	1.2	0.5	0.21
D7S1272i	6	156	20.2	13.4	0.0499	0.30	1.64
D7S2465	14	175	22.7	32.2	0.020	0.28	0.62
D7S1520i	8	300	3.2	3.0	0.58
D7S1108i	14	188	3.2	0.5	0.040	0.56	6.63
D7S1191i	6	181	0.7	0.5	0.77
D7S0968i	6	357	50.0	44.6	0.24
D7S2423	5	250	19.9	29.2	0.017	0.086	0.60
D7S1159i	8	115	13.8	9.4	0.14

Note: ^aAlleles found to exhibit the most difference in frequencies between patients and healthy participants for each marker are shown. Each allele was designated by the size of its amplification.