![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
Yang E.C., Scott J. West J.A., Orlova E., Gauthier, D., Küpper F.C., Yoon H.S. and Ulf Karsten U. 2010. New taxa of the Porphyridiophyceae (Rhodophyta): Timspurckia oligopyrenoides gen. et sp. nov. and Erythrolobus madagascarensis sp. nov. Phycologia 49: 604–616. DOI: 10.2216/09-105.1
Two new marine unicellular red algae are described: Timspurckia oligopyrenoides gen. et sp. nov. isolated from southeastern Australia and Erythrolobus madagascarensis sp. nov. isolated from Madagascar. Timspurckia oligopyrenoides cells are spherical, 7–11 µm in diameter, greyish red to reddish brown and surrounded by a conspicuous fibrillar matrix about 2 µm thick. Cells exhibit positive phototaxis. In the single chloroplast the lobes extend from several pyrenoids to occupy most of the cell. A peripheral thylakoid is absent. The pyrenoid matrices are filled with tubular thylakoids and are usually surrounded by starch sheaths in the adjacent cytoplasm. The nucleus is peripheral with a nucleolus appressed to the nuclear envelope usually closest to the cell center. Golgi bodies are associated with mitochondria usually at the cell periphery. Erythrolobus madagascarensis cells are spherical, 5–8 µm in diameter, greyish red to reddish brown and surrounded by a thin conspicuous fibrillar sheath. Cells exhibit positive phototaxis. Each cell has a single chloroplast with several lobes extending from an eccentric pyrenoid forming a complex parietal layer. A peripheral thylakoid is absent. The pyrenoid matrix is filled with tubular thylakoids, and a starch sheath is visible in the adjacent cytoplasm. The nucleus is eccentric and has a central nucleolus. Golgi bodies are associated with mitochondria usually at the cell periphery. Numerous conspicuous, electron-transparent, fibrous vesicles are always present in the cells. The sequence divergences of psaA and psbA genes and phylogeny support that E. madagascarensis sp. nov. and T. oligopyrenoides gen. et sp. nov. are newly uncovered natural entities within the class Porphyridiophyceae along with Erythrolobus coxiae, Porphyridium spp. and Flintiella sanguinaria. The low-molecular-weight carbohydrates (LMWCs) floridoside, digeneaside and trehalose are present in both taxa. Thus, three independent features – ultrastructural characters, plastid gene sequences and LMWC patterns – support the suggested phylogeny and taxonomy of these red algae.
ACKNOWLEDGMENTS
We thank Sarah Wilson for assistance with light microscopy, Chris O'Brien and Jillian Ackland for assistance with the Photoshop program and plates and Wesley Webster for help with confocal microscopy. Mark Garland provided the Latin translations. Michael Wynne and Craig Schneider advised us about the correct Latin word usage for the species, T. oligopyrenoides. Laboratory investigations have been partially supported by grants to J.A.W. from the Australian Research Council, Australian Biological Resources Study and Hermon Slade Foundation. This project also was partially supported by the National Research Foundation of Korea (Graduate Students Global Internship Program and KRF-2008-357-C00148) to E.C.Y., from the National Science Foundation Assembling the Tree of Life program to H.S.Y. (DEB-0937975), the UK Natural Environment Research Council (NERC) to F.C.K. and the CCAP (MGF 154 for sequencing and Oceans 2025 core funding) and the Deutsche Forschungsgemeinschaft (DFG) to U.K.
Corrigendum
Corrections to the paper by CitationYang E.C., Scott J., West J.A., Orlova E., Gauthier D., Küpper F.C., Yoon H.S. and Karsten U. (2010). New taxa of the Porphyridiophyceae (Rhodophyta): Timspurckia oligopyrenoides gen. et sp.nov. and Erythrolobus madagascarensis sp. nov. Phycologia 49(6): 604–616.
The designation of living cultures as the holotypes for the names of the new taxa in the above paper did not conform with Article 8.4 of the International Code of Botanical Nomenclature (CitationMcNeill et al. 2006) and resulted in those names not being validated. The purpose of this corrigendum is to validate those names.
An unrelated issue is that in the paper the copy editor for Phycologia abbreviated the generic names at the critical point where the new names were being diagnosed, which may lead to possible confusion in the citation of these taxa in the literature.
The correct full spellings of generic names are shown below:
Timspurckia oligopyrenoides gen. et sp. nov. E.C. Yang, J.L. Scott et J.A. West
The Latin diagnosis is provided in Phycologia 49: 606 (2010), and , , are given in the same article by Yang et al. cited above.
Erythrolobus madagascarensis sp. nov. E.C. Yang, J.L. Scott et J.A. West
The Latin diagnosis is provided in Phycologia 49: 607 (2010), and , are given in the same article by Yang et al. cited above.
The designation of living cultures as the holotypes of Timspurckia oligopyrenoides and Erythrolobus madagascarensis was not in conformity with Art. 8.4 of the ICBN, which reads as follows: Type specimens of names of taxa must be preserved permanently and may not be living plants or cultures. However, cultures of fungi and algae, if preserved in a metabolically inactive state (e.g. by lyophilization or deep-freezing), are acceptable as types.
Cryopreserved material is now being designated as the holotype of the two newly proposed taxa. We call attention to the fact that what was intended to as the paratype for Timspurckia oligopyrenoides in Yang et al. (2010) is now the holotype because that material is cryopreserved.
Timspurckia oligopyrenoides gen. et sp. nov. E.C. Yang, J.L. Scott et J.A. West
HOLOTYPE: Cryopreserved culture CCMP 3278, Bigelow Laboratory for Ocean Sciences, P.O. Box 475, 180McKown Point Road, West Boothbay Harbor, ME 04575, USA. This culture is JAW 3827 isolated by J. West from a mud sample obtained at the type locality: Stony Creek Backwash, Williamstown, Victoria, Australia (37° 49′S, 144° 53′E), 15 February 1998.
ISOTYPE: Dried specimen (NSW 796127) on paper from live culture JAW 3827, Royal Botanic Gardens, Mrs. Macquaries Road, Sydney, NSW 2000, Australia.
LIVE CULTURE : CCAP 1393/1 (JAW 3827), Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Scottish Marine Institute, Dunstaffnage Marine Laboratory, Dunbeg, Oban, Argyll, PA37 1QA, UK.
LIVE CULTURE: CCAP 1393/2 (JAW 4318), Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Scottish Marine Institute, Dunstaffnage Marine Laboratory, Dunbeg, Oban, Argyll, PA37 1QA, UK Victoria isolated by J. West from a mud sample obtained at the type locality: Stony Creek Backwash, Williamstown, Victoria, Australia (37° 49′S, 144° 53′E), 13 March 2003.
Erythrolobus madagascarensis sp. nov. E.C. Yang, J.L. Scott et J.A. West
HOLOTYPE: Cryopreserved culture: CCMP3276, Bigelow Laboratory for Ocean Sciences, P.O. Box 475, 180McKown Point Road, West Boothbay Harbor, ME 04575, USA, from live culture JAW4329 isolated by J. West from a culture of Sphacelaria sp. obtained on Sargassum sp. collected S. Loiseaux de Goër, Angeva, Madagascar, 23° 10.130′S, 43° 36.575′E, 22 April 2003.
ISOTYPE: Dried specimen (NSW 796126) on paper from live culture JAW 4329, Royal Botanic Gardens, Mrs. Macquaries Road, Sydney, NSW 2000, Australia.
LIVE CULTURE: CCAP 1393/3 (JAW4329). Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Scottish Marine Institute, Dunstaffnage Marine Laboratory, Dunbeg, Oban, Argyll, PA37 1QA, UK.
Many thanks to Michael J. Wynne and Paul C. Silva for pointing out errors in the original publication and for help in editing this corrigendum.
REFERENCES
- Yang, E.C., Scott, J., West, J.A., Orlova, E., Gauthier, D., Küpper, F., Yoon, H.S. & Karsten, U. 2010. New taxa of the Porphyridiophyceae (Rhodophyta): Timspurckia oligopyrenoides gen. et sp. nov. and Erythrolobus madagascarensis sp. nov. Phycologia 49: 604–616.
- McNeill, J., Barrie, F.R., Burdet, H.M., Demoulin, V., Hawksworth, D.L., Marhold, K., Nicholson, D.H., Prado, J., Silva, P.C., Skog, J.E., Wiersma, J.H. & Turland, N.J. 2006. International Code of Botanical Nomenclature (Vienna Code) adopted by the Seventeenth International Botanical Congress, Vienna, Austria, July 2005. Regnum Veg 146: 568