Phylogeny and characterisation of Nannochloropsis oceanica var. sinensis var. nov. (Eustigmatophyceae), a new oleaginous alga from China

Abstract

Cao S., Zhang X., Fan X., Qiao H., Liang C., Xu D., Mou S., Wang W. and Ye N. 2013. Phylogeny and characterisation of Nannochloropsis oceanica var. sinensis var. nov. (Eustigmatophyceae), a new oleaginous alga from China. Phycologia 52: 573–577. DOI: 10.2216/13-164.1

Strain QD001, previously identified as Chlorella, was examined using molecular phylogenetic analysis (nuclear-encoded 18S rRNA, ITS, rbcL), ITS2 secondary structure, light and electron microscopy, high-performance liquid chromatography pigment analysis, and fatty acid analysis. The strain was shown to be phylogenetically distinct from all described taxa, and the strain was named Nannochloropsis oceanica var. sinensis var. nov. DNA sequences from the new variety were very similar to GenBank sequences for N. maritima nomen nudum.

Key Words::

• 18S rRNA
• Eicosapentaenoic acid
• Fatty acid analysis
• ITS1 and ITS2
• Nannochloropsis
• Palmitoleic acid
• rbcL
• Secondary structure
• Ultrastructure
• Violaxanthin

ACKNOWLEDGEMENTS

This work was supported by Shandong Science and Technology plan project (2011GHY11528), the Specialized Fund for the Basic Research Operating Expenses Program (20603022012004), National Natural Science Foundation of China (41176153), Natural Science Foundation of Shandong Province (2009ZRA02075), Qingdao Municipal Science and Technology plan project (11-3-1-5-hy) and National Marine Public Welfare Research Project (200805069).

SUPPLEMENTARY DATA

Supplementary data associated with this article can be found online at http://dx.doi.org/10.2216/13-164.1.s1.