RAS Mutation Prevalence Among Patients With Metastatic Colorectal Cancer: a Meta-Analysis of Real-World Data

Figures & data

Table 1.  Data sources included in the analysis.

Data source	Country	Type of data source	Study period	Number of samples^†
Pathology & clinical data
Argentina	Argentina	Amgen-sponsored study	March 2014 to November 2014	565
Czech Republic I	Czech Republic	Pathology center	January 2014 to December 2014	100
Greece	Greece	Amgen-sponsored study	December 2013 to August 2014	514
Hungary I	Hungary	Pathology center	September 2013 to June 2014	150
Hungary II	Hungary	Pathology center	September 2013 to June 2014	150
Middle East Registry	Algeria, Egypt, Saudi Arabia, UAE, Lebanon, Kuwait, Bahrain	mCRC registry	2013 to 2014	188
Pathology-only data
Czech Republic II	Czech Republic	Pathology center	January 2014 to December 2014	100
Czech Republic III	Czech Republic	Pathology center	January 2014 to December 2014	100
Middle East Pathology I	Algeria, Saudi Arabia, UAE, Lebanon, Kuwait, Bahrain	mCRC registry	2012 to 2015	447
Middle East Pathology II	Egypt	mCRC registry	2012 to 2015	303
Poland I	Poland	Pathology center	January 2014 to December 2014	1664
Poland II	Poland	Pathology center	January 2014 to December 2014	150

^†Patients may have had more than one sample taken and tested for RAS mutation status, and therefore may be represented in the aggregate data more than once.

mCRC: Metastatic colorectal cancer.

Figure 1.  Overall RAS mutation prevalence.

Forest plot showing biomarker prevalence by data source.

mCRC: Metastatic colorectal cancer.

Table 2.  Pooled RAS prevalence estimates by patient characteristics.

Variable	Subgroups	Pooled RAS prevalence estimate (%)	95% CI	Number of data sources included in analysis
Overall RAS mutation prevalence		43.6	38.8–48.5	12
Age (years)	18–49	42.9	29.7–56.5	6
	50–69	40.9	33.9–48.2	12
	18–69	42.1	36.8–47.5	12
	≥70	49.6	44.8–54.3	10
Gender	Female	43.3	36.8–49.9	10
	Male	43.8	39.0–48.6	12
Tissues from which DNA was isolated	Left colon	42.7	37.8–47.6	9
	Right colon	45.5	35.5–55.7	9
	Rectum	44.2	38.1–50.3	11
Site tumor sample was taken from	Primary	43.3	36.9–49.9	9
	Metastatic	51.3	43.0–59.5	3
Liver metastases^†	Liver metastasis	39.7	30.3–49.4	7
	No liver metastasis	43.2	36.5–50.1	6
Metastatic development	Synchronous	47.5	40.1–55.0	5
	Metachronous	42.0	32.4–51.9	6
Tumor stage^†	Stage I/II	44.0	33.5–54.7	5
	Stage III	40.4	32.4–48.6	5
	Stage IV	43.7	35.8–51.8	6
Line of treatment^†	1st line	49.3	40.7–57.8	2
	2nd line	35.8	31.2–40.6	2
	3rd line or later	39.2	33.4–45.1	2
Ratio of neoplastic cells as a percentage	<20%	43.0	33.3–52.9	2
	≥20%	47.0	42.6–51.4	9

^†Subgroups were based on patient status at the time the tumor sample was taken.

Figure 2.  Overall BRAF mutation prevalence.

Figure 3.  Overall non-KRAS exon 2 RAS mutation prevalence, in patients with a confirmed wild-type KRAS exon 2 status.

Table 3.  RAS mutation testing turnaround time by data source.

Data source	Number of samples	Turnaround time (working days), n (%)
		≤5	6–10	>10
Czech Republic I	100	37 (37.0)	53 (53.0)	10 (10.0)
Czech Republic II	100	43 (43.0)	47 (47.0)	10 (10.0)
Czech Republic III	100	56 (56.0)	39 (39.0)	5 (5.0)
Greece	510	0 (0)	510 (100.0)	0 (0)
Hungary I	150	37 (24.7)	113 (75.3)	0 (0)
Hungary II	150	46 (30.7)	73 (48.7)	31 (20.7)
Middle East Pathology I	447	262 (58.6)	185 (41.4)	0 (0)
Middle East Pathology II	303	274 (90.4)	29 (9.6)	0 (0)
Middle East Registry	158	33 (20.9)	61 (38.6)	64 (40.5)
Poland I	1664	1368 (82.2)	274 (16.5)	22 (1.3)
Poland II	150	27 (18.0)	71 (47.3)	52 (34.7)
Overall	3832	2183 (57.0)	1455 (38.0)	194 (5.1)