A Functional Polymorphism in the Promoter Region of IL-33 Is Associated with the Reduced Risk of Colorectal Cancer

Abstract

Aim: We aimed to investigate IL-33 polymorphisms with risk of colorectal cancer (CRC). Materials & methods:IL-33 rs7025417 and rs1332290 were genotyped using a quantitative allelic Taqman assay. The expression of IL-33 mRNA was determined by real-time PCR and promoter activity was assayed using the Dual-Luciferase Reporter Assay. Results: The IL-33 rs7025417 CC genotype and C allele may decrease CRC risk. The IL-33 rs1332290 AC carriers had an increased risk of developing clinical Stage III–IV CRC. Lower levels of IL-33 mRNA were present in individuals with the rs7025417 CC genotype. Moreover, the rs7025417 C allele suppressed promoter activity of IL-33. Conclusion: These data suggest that the rs7025417 CC genotype may downregulate IL-33 mRNA and subsequently reduce the risk of CRC.

Keywords:

• colorectal cancer
• IL-33
• luciferase activity
• polymorphism

Financial & competing interests disclosure

This work was supported by the Luoyang Bureau of Science and Technology (grant number 1721001A-6). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Funded writing assistance was supported by (grant number 1721001A-6).

Ethical conduct of research

All procedures performed in studies involving human participants were in accordance with the ethical standards of Zhengzhou University and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.

Additional information

Funding

This work was supported by the Luoyang Bureau of Science and Technology (grant number 1721001A-6). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.