Analysis of FUSOBACTERIUM NUCLEATUM and Streptococcus Gallolyticus in Saliva of Colorectal Cancer Patients

Abstract

Aim: The aim of the study was to examine the prevalence and amount of Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg) and Streptococcus gallolyticus (Sg) in the saliva of colorectal cancer (CRC) patients and controls. Methods: PCR analyses performed in 71 CRC patients and 77 controls. Results: Saliva samples of patients had higher amounts of Fn (p = 0.001) and Sg (p < 0.001) compared with controls. Amount of Fn and Sg were lower in the microsatellite instability (+) group. Evaluation of salivary Sg amount by receiver operating characteristics analysis found to have diagnostic value for CRC (AUC: 0.84, 95% CI: 0.72–0.96). Conclusion: We found higher amounts of Fn and Sg in the saliva of CRC patients. Salivary Sg could helpful in distinction of CRC.

Keywords:

• colorectal cancer
• Fusobacterium nucleatum
• oral microbiome
• Streptococcus gallolyticus

Acknowledgements

We thank the H Cebbari of BM Laboratories for his great support on microbiological analyses of this study.

Financial & competing interests disclosure

This study was funded by Hacettepe University Scientific Research Projects Coordination Unit. Project number: THD-2017-12288. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The study was approved by the Ethics committee of Hacettepe University with the approval number of GO16/434-19. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

This study was funded by Hacettepe University Scientific Research Projects Coordination Unit. Project number: THD-2017-12288. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.