Potential Clinical Significance of ALDH3B1 in Auxiliary Diagnosis of Gastric Cancer

Figures & data

Figure 1. Evaluation of the method of detection.

(A & D) The effect of different freeze–thaw cycles on the expression of ALDH3B1 and 18S. (B & E) The effect of different Incubation duration on the expression of ALDH3B1 and 18S. (C & F) The effect of different dilution factor on the expression of ALDH3B1 and 18S.

18S: 18S rRNA.

Figure 2. Expressions of serum aldehyde dehydrogenase 3B1 in thyroid cancer, colorectal cancer, breast cancer and gastric cancer patients.

(A) Differential expression of ALDH3B1 in the serum of thyroid cancer patients and healthy donors. (B) Differential expression of ALDH3B1 in the serum of colorectal cancer patients and healthy donors. (C) Differential expression of ALDH3B1 in the serum of breast cancer patients and healthy donors. (D) Differential expression of ALDH3B1 in the serum of gastric cancer patients and healthy donors.

Figure 3. Diagnostic efficacy of aldehyde dehydrogenase 3B1.

(A) Differential expression of ALDH3B1 in the serum of GC patients (n = 76) and healthy donors (n = 70). (B) Combined diagnostic of serum ALDH3B1 and CEA exerted the best diagnostic efficacy in distinguishing GC patients and healthy donors (AUC = 0.841). (C) ROC curve analysis of serum ALDH3B1 for discriminating GC patients and healthy donors (AUC = 0.817). (D) The diagnostic efficacy of CEA (AUC = 0.698).

AUC: Area under curve; CEA: Carcinoembryonic protein; GC: Gastric cancer; ROC: Receiver operating characteristic.

Table 1. Clinicopathological analysis of serum aldehyde dehydrogenase.

Parameter		Patients (n)	ALDH3B1 (high)	ALDH3B1 (low)	p-value
Sex	Male	44	31	13	0.655
	Female	32	21	11
Age (years)	<60	19	14	5	0.665
	≥60	57	39	18
Tumor size	<5	43	29	14	0.273
	≥5	33	26	7
Differentiation grade	Well–moderate	44	31	13	0.662
	Poor–undifferentiated	32	24	8
T stage	T1–T2	40	25	15	0.008^‡
	T3–T4	36	32	4
Lymph node status	Positive	29	16	13	0.03^†
	Negative	47	37	10
TNM stage	I–II	30	17	13	0.045^†
	II–IV	46	36	10
Nerve/vascular invasion	Positive	36	28	8	0.442
	Negative	40	28	12

† p < 0.05.

‡ p < 0.01.

T stage: Tumor stage; TNM: Tumor–node–metastasis.

Figure 4. Monitoring capacity of serum aldehyde dehydrogenase 3B1.

(A) Differential expression of ALDH3B1 in the serum of patients with benign lesions and healthy donors. (B) Differential expression of ALDH3B1 in the serum of postoperative GC patients and healthy donors. (C) Differential expression of ALDH3B1 in the serum of recurrent GC patients and healthy donors. (D) ALDH3B1 protein was highly expressed in three pairs of GC tissues compared to normal gastric tissues.

GC: Gastric cancer. N: Normal gastric tissue; T: GC tumors.