Identical Twins Doubly Exchanged at Birth: A Case Report of Genetic and Environmental Influences on the Adult Epigenome

Figures & data

Figure 1.  Unsupervised cluster diagram of data from 65 SNP probes from the twins and sister.

These probes are highly polymorphic SNPs, intended for analyzing relatedness between individuals. The y-axis represents arbitrary units of differences between individuals. Monozygotic pairs are coded as in the text.

Figure 2.  Cluster diagram of beta values of 150,678 probes of the twins.

Individuals are labeled the same way as Figure 1.

Figure 3.  Multidimensional scaling plots of principal components (A) 1 versus 2 and (B) 3 versus 4.

These are based on M values of 150,678 probes. Although we have a low sample size, genetics appears to be best represented by PC1 and rearing environment by PC3. Individuals are labeled the same say as Figure 1.

Table 1.  Between individual intraclass correlation coefficients analysis of twins across 150,678 probes.

G2E2	G1E2	G2E1	S(G1E1)
0.987	0.989	0.989	0.989	G1E1
	0.991	0.990	0.990	G2E2
		0.991	0.992	G1E2
			0.991	G2E1

Table 2.  REVIGO terms with log10p value less than -1.3 and associated GO terms.

GO term^†	GO description^†	REVIGO terms	Genes^‡
GO:0006955	Immune response	Immune response	CD300LB, HLA-L
GO:0006952	Defense response	Immune response	GABRA5, CXCR2
GO:0006461	Protein complex assembly	Protein complex assembly	–
GO:0070271	Protein complex biogenesis	Protein complex assembly	–
GO:0008219	Cell death	Cell death	CXCR2, TNFRSF10C
GO:0016265	Death	Death	CXCR2, TNFRSF10C
GO:0010605	Negative regulation of macromolecule metabolic process	Negative regulation of macromolecule metabolism	–
GO:0006351	Transcription, DNA-templated	Negative regulation of macromolecule metabolism	–
GO:0007166	Cell surface receptor signaling pathway	Immune response	GABRA5, PROKR1, CXCR2

^†GO terms and descriptions in italics have a false discovery rate < 0.05 in DAVID.

^‡Genes that have 2 or more top 1000 probes annotated, and associated with GO terms with false discovery rate < 0.05 values found in REVIGO terms with log10 p < -1.3.