A Novel Discriminating Colorectal Cancer Model for Differentiating Normal and Tumor Tissues

Abstract

Aim: To construct a model discriminating colorectal cancer (CRC) based on differential DNA methylation. Materials & methods: The CRC-related methylation-modulated genes were retrieved from literature. The methylation levels of CpG sites in the promoter regions and the first exons of candidate genes were verified in The Cancer Genome Atlas data. A model for discriminating CRC based on methylation levels was established using least absolute shrinkage and selection operator regression. Results: Five new differentially methylated CpG sites were identified and further validated in 94 Chinese CRC patients. A five-CpG-based panel was constructed, with the area under the curve values of 0.999 in The Cancer Genome Atlas data and 0.943 in Chinese patients, respectively. Conclusion: This five-CpG-based panel is a practical and reliable tool for CRC discrimination.

Keywords::

• colorectal cancer
• diagnostic value
• DNA methylation

Supplementary data

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Financial & competing interests disclosure

This work was supported by the grants from National Key Research and Development Program of China (grant number: 2017YFC0907004); the Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents and the Fundamental Research Funds for the Central Universities 2012-141. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

This study was approved by the research ethics committees of Zhejiang University School of Medicine (number: 2012-1-012). The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

This study was approved by the research ethics committees of Zhejiang University School of Medicine (number: 2012-1-012). The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.