Abstract
Aim: To investigate the antibacterial effects of Corydalis Saxicola bunting total alkaloid (CSBTA) on Porphyromonas gingivalis. Methods: SEM, chemical staining, RT-qPCR and ELISA were used to detect effects of CSBTA on P. gingivalis. Results: CSBTA treatment caused shrinkage and rupture of P. gingivalis morphology, decreased biofilm density and live bacteria in biofilm, as well as reduced mRNA expression of virulence genes hagA, hagB, kgp, rgpA and rgpB of P. gingivalis. Furthermore, NOK cells induced by CSBTA-treated P. gingivalis exhibited lower IL-6 and TNF-α expression levels. Conclusion: CSBTA is able to kill free P. gingivalis, disrupt the biofilm and weaken the pathogenicity of P. gingivalis. It has the potential to be developed as a drug against P. gingivalis infection.
Corydalis Saxicola bunting total alkaloid (CSBTA) exhibits effectively antibacterial activity against Porphyromonas gingivalis in vitro.
CSBTA is able to kill the free Porphyromonas gingivalis and destroy its biofilm.
CSBTA weaken the pathogenicity of Porphyromonas gingivalis.
CSBTA may have potential as a treatment for Porphyromonas gingivalis infection.
Author contributions
W Ye: methodology, statistical analysis and original draft. J Wu: experiments and statistical analysis. Q Jiang: experiments and statistical analysis. Z Su: methodology and data curation. H Liao: experiments and data collection. Z Liu: experiments. R Tao: supervision, study conception and coordination and project administration. X Yong: study design, methodology, experiments, review & editing, statistical analysis and project administration. All the authors have read the final manuscript and approved the submission.
Financial disclosure
This research was supported by grants from General Project of Guangxi Natural Science Foundation (2023GXNSFAA026309), Youth Science Foundation Project of Guangxi Natural Science Foundation (2020GXNSFBA297159), National Natural Science Foundation of China (82160180) and Guangxi Medical High-level Talents Training Program. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
Competing interests disclosure
The authors have no competing interests or relevant affiliations with any organization or entity with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.
Writing disclosure
No writing assistance was utilized in the production of this manuscript.