Hemostatic and Hepatoprotective Bioactivity of Junci Medulla Carbonisata-Derived Carbon Dots

Abstract

Aim: To explore the hemostatic and hepatoprotective bioactivity of Junci Medulla Carbonisata-derived Carbon Dots (JMC-CDs). Materials & methods: The JMC-CDs were characterized using transmission electron microscopy, HPLC, Fourier transform IR, UV, fluorescence and x-ray photoelectron spectroscopy. The hemostatic effect of JMC-CDs was evaluated and confirmed by trauma hemorrhagic animal models and internal hemorrhage animal model induced by Deinagkistrodon acutus venom. Results: The JMC-CDs ranged in diameter from 1.0 to 8 nm and had a yield of 0.12%. Moreover, JMC-CDs not only possessed remarkable hemostatic efficacy but could also prevent hemorrhage-induced liver injury, as demonstrated by the reduced serum levels of biochemical indicators of liver damage such as aspartate aminotransferase, alanine amino transferase, alkaline phosphatase, total bilirubin and direct bilirubin. Conclusion: The JMC-CDs may have great potentials in clinical practice.

Keywords::

• carbon dots
• Deinagkistrodon acutus
• hemostatic bioactivity
• Junci Medulla Carbonisata
• liver protection

Financial & competing interests disclosure

We greatly appreciate the support of the National Natural Science Foundation (grant numbers 81573573, 81503344 and 81473338) and the Classical Prescription Basic Research Team of the Beijing University of Chinese Medicine. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

We greatly appreciate the support of the National Natural Science Foundation (grant numbers 81573573, 81503344 and 81473338) and the Classical Prescription Basic Research Team of the Beijing University of Chinese Medicine. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.