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Research Article

Approaches to measurement of Vitamin D concentrations – Mass spectrometry

Pages 54-59 | Published online: 26 Apr 2012

Figures & data

Figure 1. Schematic drawing of tandem-mass spectrometry.

Figure 1. Schematic drawing of tandem-mass spectrometry.

Figure 2. HPLC-MS/MS separation for a serum sample containing a) 25(OH)D3, b) 3-epi-25(OH)D3, c) 25(OH)D2, d) 24,25(OH)2D3 and e) d6-25(OH)D3, internal standard.

Figure 2. HPLC-MS/MS separation for a serum sample containing a) 25(OH)D3, b) 3-epi-25(OH)D3, c) 25(OH)D2, d) 24,25(OH)2D3 and e) d6-25(OH)D3, internal standard.

Figure 3. Concentration of epi25OHD3 and 24,25(OH)2D3 relative to 25(OH)D3 concentration in a set of patient samples.

Figure 3. Concentration of epi25OHD3 and 24,25(OH)2D3 relative to 25(OH)D3 concentration in a set of patient samples.

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