Figures & data
Figure 2. Potentiation of enediyne toxicity by hyperthermia. HeLa CCL-2 cells were treated with various concentrations of either PyED or QuinED · ZnCl2 for 1 h at 37°C or 42.5°C. Surviving fraction was normalised to vehicle only (1.5% DMSO) for each respective temperature. Error bars represent SEM (n = 2 for drug curves and n = 4 for DMSO only curves). (A) PyED; (B) QuinED · ZnCl2.
![Figure 2. Potentiation of enediyne toxicity by hyperthermia. HeLa CCL-2 cells were treated with various concentrations of either PyED or QuinED · ZnCl2 for 1 h at 37°C or 42.5°C. Surviving fraction was normalised to vehicle only (1.5% DMSO) for each respective temperature. Error bars represent SEM (n = 2 for drug curves and n = 4 for DMSO only curves). (A) PyED; (B) QuinED · ZnCl2.](/cms/asset/071c0e41-0f04-4b7a-9f80-e09d5e9376e1/ihyt_a_578607_f0002_b.gif)
Figure 3. Alterations in the mode of death after treatment with PyED or QuinED · ZnCl2 for 1 h at 37°C or 42.5°C. Apoptosis was measured using the Annexin V/PI assay as described in the Materials and methods section. (A) Bivariate plots of PI (y-axis) versus Annexin V-EFGP (x-axis) fluorescence. HeLa CCL-2 cells were incubated with DMSO or drugs for 1 h at 37°C or 42.5°C for 1 h and fixed 24 h after incubation. Percentage of cells in lower right quadrant was taken as the apoptotic fraction. (B) Apoptotic fractions obtained from bivariate plots were plotted as a function of time for each enediyne treatment. Y-error bars represent SEM (n = 2 for drug curves and n = 4 for DMSO only curves).
![Figure 3. Alterations in the mode of death after treatment with PyED or QuinED · ZnCl2 for 1 h at 37°C or 42.5°C. Apoptosis was measured using the Annexin V/PI assay as described in the Materials and methods section. (A) Bivariate plots of PI (y-axis) versus Annexin V-EFGP (x-axis) fluorescence. HeLa CCL-2 cells were incubated with DMSO or drugs for 1 h at 37°C or 42.5°C for 1 h and fixed 24 h after incubation. Percentage of cells in lower right quadrant was taken as the apoptotic fraction. (B) Apoptotic fractions obtained from bivariate plots were plotted as a function of time for each enediyne treatment. Y-error bars represent SEM (n = 2 for drug curves and n = 4 for DMSO only curves).](/cms/asset/a7ffec62-c9fa-4c3a-bb16-e42bc55a5aca/ihyt_a_578607_f0003_b.gif)
Figure 4. Perturbation of the cell cycle in cells treated with enediynes and hyperthermia. Shown are representative DNA distributions accumulated for HeLa CCL-2 cells incubated with DMSO only or either PyED or QuinED · ZnCl2 for 1 h at 37°C or 42.5°C and fixed 16 h after incubation prior to staining with propidium iodide and analysis by flow cytometry.
![Figure 4. Perturbation of the cell cycle in cells treated with enediynes and hyperthermia. Shown are representative DNA distributions accumulated for HeLa CCL-2 cells incubated with DMSO only or either PyED or QuinED · ZnCl2 for 1 h at 37°C or 42.5°C and fixed 16 h after incubation prior to staining with propidium iodide and analysis by flow cytometry.](/cms/asset/d0650c85-9bba-4ad4-a430-6ec46a9b3037/ihyt_a_578607_f0004_b.gif)
Figure 5. Alterations in cell cycle progression of cells treated with enediynes and hyperthermia. The fraction of cells in each phase of the cell cycle was plotted as a function of time after drug (PyED or QuinED · ZnCl2) and heat treatments. Y-error bars represent SEM (n = 2 for drug curves and n = 4 for DMSO only curves).
![Figure 5. Alterations in cell cycle progression of cells treated with enediynes and hyperthermia. The fraction of cells in each phase of the cell cycle was plotted as a function of time after drug (PyED or QuinED · ZnCl2) and heat treatments. Y-error bars represent SEM (n = 2 for drug curves and n = 4 for DMSO only curves).](/cms/asset/d706abc7-a0a1-4e7a-bb8b-b8c786ef1c8a/ihyt_a_578607_f0005_b.gif)