Melan-A specific CD8+ T lymphocytes after hyperthermic isolated limb perfusion: A pilot study in patients with in-transit metastases of malignant melanoma

Figures & data

Table I. Patient characteristics.

Patient	Age	Gender	Local	Vessel	N-stage
1	63	Female	Foot	Femoral	N3
2	59	Male	Lower arm	Axillary	N2c
3	92	Female	Lower leg	Femoral	N2c
4	68	Female	Foot	Femoral	N3
5	87	Male	Lower leg	Femoral	N2c
6	73	Male	Foot	Femoral	N3
7	76	Female	Lower leg	Iliacal	N2c
8	63	Male	Lower leg	Iliacal	N3
9	53	Female	Lower arm	Axillary	N3
10	81	Male	Whole leg	Femoral	N3
11	72	Male	Whole leg	Femoral	N3
12	78	Female	Whole leg	Femoral	N3

Figure 1. FACS plot of Melan-A/Mart-1 specific CD8+ T-cells (Patient 1) using phycoerythrin conjugated ELAGIGTV-pentamer. The results show an increase from 1.09% before isolated limb perfusion to 6.08% after 4 weeks.

Figure 2. Levels (mean ± SD) of different lymphocyte subpopulations before and after (1 week, 4 weeks and 3 months) isolated limb perfusion. No significant changes were observed.

Figure 3. (a) Of the analyzed subpopulations in Figure 2, the levels of CD3+8+, CD3+8+45RA+ and CD3+DR+ cells were significant for clinical response. (b) Levels of Melan-A specific CD8+ T-cells during 12 weeks follow-up after isolated limb perfusion.