Figures & data
Figure 1. Preparation of HSP-fusion vaccine from CSC. The tumour is depicted as a colony of cells containing CSC (spindle/mesenchymal shape) and more differentiated cells (cuboid shape). To prepare vaccine, cells are disaggregated and CSC are sorted by cell surface phenoptype (CD44+ CD24−) using fluorescence-labelled monoclonal antibodies and cell sorting by fluorescence activated cell sorting. CSC are then fused to autologous DC by the polyethylene glycol approach as described in Wang et al. [Citation19], leading to formation of fusion cells. Fusion cells can be used as vaccine in this state or lysed and the HSP fusion vaccine is prepared using Hsp70 antibody immunoaffinity chromatography as in Wang et al. [Citation19].
![Figure 1. Preparation of HSP-fusion vaccine from CSC. The tumour is depicted as a colony of cells containing CSC (spindle/mesenchymal shape) and more differentiated cells (cuboid shape). To prepare vaccine, cells are disaggregated and CSC are sorted by cell surface phenoptype (CD44+ CD24−) using fluorescence-labelled monoclonal antibodies and cell sorting by fluorescence activated cell sorting. CSC are then fused to autologous DC by the polyethylene glycol approach as described in Wang et al. [Citation19], leading to formation of fusion cells. Fusion cells can be used as vaccine in this state or lysed and the HSP fusion vaccine is prepared using Hsp70 antibody immunoaffinity chromatography as in Wang et al. [Citation19].](/cms/asset/388f62bb-9f28-4f0d-97f0-1b9bce7eb939/ihyt_a_792126_f0001_b.jpg)