Figures & data
Table 1. Particle size and Zeta potential of nanoparticles.
Figure 1. Transmission electron micrographs of nanoparticles: (A) 5-FU-loaded PLGA-coated nanoparticles with iron core, (B) PLGA-coated nanoparticles without iron core.
![Figure 1. Transmission electron micrographs of nanoparticles: (A) 5-FU-loaded PLGA-coated nanoparticles with iron core, (B) PLGA-coated nanoparticles without iron core.](/cms/asset/93fa22c4-e14f-4872-b1cd-36531ab69c53/ihyt_a_1035766_f0001_b.jpg)
Figure 2. In vitro release profiles of 5-FU from 5-FU-loaded PLGA-coated nanoparticles with/without iron core. The plot represents mean ± SD of triplicate results.
![Figure 2. In vitro release profiles of 5-FU from 5-FU-loaded PLGA-coated nanoparticles with/without iron core. The plot represents mean ± SD of triplicate results.](/cms/asset/d53a4154-23c1-47bf-a06f-4d9504fb0c54/ihyt_a_1035766_f0002_b.jpg)
Figure 3. The images confirm the entry of nanoparticles into the cell: (A) control image, (B) image with nanoparticles.
![Figure 3. The images confirm the entry of nanoparticles into the cell: (A) control image, (B) image with nanoparticles.](/cms/asset/c96efcc9-d555-4ed3-a298-1b5be8c03406/ihyt_a_1035766_f0003_c.jpg)
Figure 4. Effects of different concentrations of 5-FU and hyperthermia on (A) 5-FU-loaded PLGA-coated nanoparticles without iron oxide core, (B) 5-FU-loaded PLGA-coated nanoparticles with iron core with hyperthermia, and (C) the viability of HT-29 spheroid culture cells. The viability was measured using trypan blue dye exclusion assay method. Mean ± SEM of three experiments.
![Figure 4. Effects of different concentrations of 5-FU and hyperthermia on (A) 5-FU-loaded PLGA-coated nanoparticles without iron oxide core, (B) 5-FU-loaded PLGA-coated nanoparticles with iron core with hyperthermia, and (C) the viability of HT-29 spheroid culture cells. The viability was measured using trypan blue dye exclusion assay method. Mean ± SEM of three experiments.](/cms/asset/819e5a9b-f48e-48a4-a339-cc925525aa93/ihyt_a_1035766_f0004_b.jpg)
Figure 5. Effects of different concentrations of 5-FU with and without hyperthermia on induced DNA damage of HT-29 spheroid culture cells. Mean ± SEM of three experiments.
![Figure 5. Effects of different concentrations of 5-FU with and without hyperthermia on induced DNA damage of HT-29 spheroid culture cells. Mean ± SEM of three experiments.](/cms/asset/5d2d5851-ad51-4ad0-bf59-4c39cdc73329/ihyt_a_1035766_f0005_b.jpg)
Figure 6. Effects of different concentrations of 5-FU or 5-FU-loaded nanoparticles with and without iron core on induced DNA damage of HT-29 spheroid culture cells. Mean ± SEM of three experiments.
![Figure 6. Effects of different concentrations of 5-FU or 5-FU-loaded nanoparticles with and without iron core on induced DNA damage of HT-29 spheroid culture cells. Mean ± SEM of three experiments.](/cms/asset/6d47f207-321f-4e77-8160-0c0de164af38/ihyt_a_1035766_f0006_c.jpg)
Figure 7. Effects of 5-FU-loaded PLGA-coated iron-free nanoparticles with and without hyperthermia on induced DNA damages of HT-29 spheroid culture cells. Mean ± SEM of three experiments.
![Figure 7. Effects of 5-FU-loaded PLGA-coated iron-free nanoparticles with and without hyperthermia on induced DNA damages of HT-29 spheroid culture cells. Mean ± SEM of three experiments.](/cms/asset/a48a3137-4c34-4ee8-95ae-45c91413744d/ihyt_a_1035766_f0007_b.jpg)