Immunohistochemical biomarkers and FDG uptake on PET/CT in head and neck squamous cell carcinoma

Figures & data

Figure 1. IHC vs TSUVmax. Immunohistochemical stains versus TSUVmax. The proportion of positive cells have been binned in three groups, low expression (up to 1% positively stained cells) intermediate expression (> 1–33% positively stained cells) and high expression (> 33% positively stained cells) in order to have sufficient numbers in each stratum. p16 is scored dichotomously according to EORTCs p16 scoring guidelines [18].

Table I. Results of univariate and multivariate linear regression.

	TSUVmax
	Univariate analysis					Multivariate analysis
	B	95% CI	p	r	r-squared	B	95% CI	p	r	r-squared
Bcl-2	−1.12	−1.81; −0.43	0.002	0.32	0.10	−0.77	−1.47; −0.07	0.032	0.51	0.26
p53	−0.27	−1.05; 0.51	0.499	0.07	0.01	–	–	–
GST-π	0.91	−0.44; 2.25	0.184	0.14	0.02	–	–	–
β-tubulin-1	2.04	0.69; 3.38	0.003	0.30	0.09	2.04	0.52; 3.56	0.009
β-tubulin-2	−1.14	−2.56; 0.27	0.112	0.17	0.03	–	–	–
p16 positivity	−3.84	−6.46; −1.22	0.005	0.29	0.08	−2.97	−5.69; −0.24	0.033
Ki-67	−0.95	−2.19; 0.29	0.133	0,16	0.03	–	–	–
EGFR	0.99	−0.15; 2.1	0.088	0.18	0.03	–	–	–

Figure 2. Proportion of p16 positive tumors. Logistic regression analysis of the probability of p16 positivity as a function of TSUVmax. Mean TSUVmax (with exact binomial confidence intervals) plotted for five 20 percentile groups based on TSUVmax. The knowledge of the TSUVmax of a tumor can be used to give a probability of the tumor being p16 positive. Logistic regression equation for p16: P = (exp(0.543+−0.108*TSUVmax))/(1 + exp(0.543+−0.108*TSUVmax)).

Figure 3. Proportion of Bcl-2 positive tumors. Logistic regression analysis of function with the probability of Bcl-2 positivity as a function of TSUVmax. Mean TSUVmax (with exact binomial confidence intervals) plotted for five 20 percentile groups based on TSUVmax. The knowledge of the TSUVmax of a tumor can be used to give a probability of the tumor being Bcl-2 positive. Logistic regression equation for Bcl-2: P = (exp(1.467+−0.103*TSUVmax))/(1 + exp(1.467+−0.103*TSUVmax)).

Lassen P. DAHANCA/EORTC guidelines for scoring and classification of p16-immunohistochemistry in HPV-related oropharyngeal cancer 2012. [cited 2015 May 10]. Available from: http://www.dahanca.dk/get_media_file.php?mediaid = 322

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