Differential Expression of Wound Fibrotic Factors between Facial and Trunk Dermal Fibroblasts

Figures & data

Table 1. Profiles and data description codes of samples

No.	Age	Sex	Proliferation assay	Real-time PCR assay	ELISA (collagen I)	ELISA (cytokines)	Symbol in figures
1	44	M	*	*	*	*	○
2	34	M	*	*	*	*	▵
3	60	F	*	*	*	*	□
4	65	M	*	*	*	*	•
5	53	F	*	*	*	*	▴
6	24	F	*	*		*	▪
7	46	F	*	*			×

Table 2. Primer sequences and optimized concentrations for real-time PCR

Gene	Coding protein	Sequence	Primer concentration [nM]
COL1A1	Type I collagen	F: CCCACCAATCACCTGCGTACAGA	100
		R: TTCTTGGTCGGTGGGTGACTCTGA	100
COL3A1	Type III collagen	F: GAGATGTCTGGAAGCCAGAACCAT	100
		R: GATCTCCCTTGGGGCCTTGAGGT	100
FN1	Fibronectin	F: GGAGAATTCAAGTGTGACCCTCA	300
		R: TGCCACTGTTCTCCTACGTGG	300
MMP1	MMP1	F: TCTGGGGTGTGGTGTCTCA	300
	(collagenase)	R: GCCTCCCATCATTCTTCAGGTT	300
TGFB1	TGF-β1	F: GTTCAAGCAGAGTACACACAGC	300
		R: GTATTTCTGGTACAGCTCCACG	300
TGFB2	TGF-β2	F: ATGCGGCCTATTGCTTTAGA	200
		R: TAAGCTCAGGACCCTGCTGT	200
TGFB3	TGF-β3	F: CAGGGAGAAAATCCAGGTCA	100
		R: CCTGGAAGGCGTCTAACCAAG	100
CTGF	CTGF	F: ACGGCGAGGTCATGAAGAAGAACA	100
		R: ACTCTCTGGCTTCATGCCATGTCT	100
ASMA	α-smooth muscle actin	F: CCAAGCACTGTCAGGAAT	100
		R: AGGCAGTGCTGTCCTCTT	100
FGF2	bFGF	F: ATACAGCAGCAGCCTAGCAACTCT	100
		R: TTCGGCAACAGCACACAAATCCTG	100
HGF	HGF	F: GCAAGTGAATGGAAGTCCTTTA	100
		R: CAGAGGGACAAAGGAAAAGAA	100
SCF	SCF	F: GCCGCTGTTCGTGCAATAT	200
		R: CTGCGATCCAGCACAAACAGT	200
HARP	HARP	F: CGCTGCTGAACATGCTCAA	300
		R: TGTCGAACACCTGCTGGATG	300
GAPDH	GAPDH	F: GAAGGTGAAGGTCGGAGTC	300
		R: GAAGATGGTGATGGGATTTC	300

Figure 1. Cell morphology of FS, TS, FD, and TD. Phase contrast microscopic findings for FS, TS, FD, and TD from donor No. 4 at 4, 12, and 32 days after cell seeding. Scale bar indicates 100 μm.

Figure 2. Cell proliferation of FS, TS, FD, and TD. (A) Chronological cell number in four cell fractions is noted (n = 7 for each). Error bars indicate SEM. (B) Cell count of FS, TS, FD, and TD on day 32 (n = 7 for each). (C) Correlation of saturated cell number between facial and trunk fibroblasts on day 32.

Figure 3. Expression of wound healing-associated factors by FS, TS, FD, and TD. mRNA expression of fibrosis-associated factors by FS, FD, TS, and TD (n = 7, each).

Figure 4. Production of wound healing-associated factors by FS, TS, FD, and TD. Production of type I collagen, TGF-β1 and TGF-β2, and CTGF by FS, FD, TS, and TD (for type I collagen, n = 5 for each; for others, n = 6 for each).