Figures & data
Figure 1. Expression of FOXM1 in NPC and normal nasopharyngeal tissues (NT). A: Overexpression of FOXM1 in NPC and low expression in NT as evidenced by immunohistochemical staining. B: Relative FOXM1 mRNA levels in NPC and NT were determined by qRT-PCR. C: Overexpression of FOXM1 in NPC and low expression in NT were determined by Western blot. Experiments were independently repeated three times. *p < 0.05.
Table I. Expression of FOXM1 in NPC and normal tissue specimens.
Table II. Correlation between the clinicopathologic features and expression of FOXM1.
Figure 2. Thiostrepton significantly reduces the expression of FOXM1 mRNA and protein in a NPC cell line other than a nasopharyngeal epithelial cell line. A: The expression of FOXM1 mRNA in C666-1 cells was inhibited after thiostrepton treatment at 2, 4, or 6 µM for 48 h. B: Thiostrepton down-regulated the expression of FOXM1 protein in C666-1 cells. C: The expression of FOXM1 mRNA in NP69 cells was lower than in C666-1 cells and was less affected by thiostrepton. Experiments were independently repeated three times. *p < 0.05.
Figure 3. Thiostrepton reduces the invasion and migration activities of NPC cells. A: Invasion and migration assays were used to detect the invasion and migration ability of C666-1 cells following treatment with 2, 4, or 6 µM thiostrepton for 48 h. B: Manual counts of invasion and migration experiments. Experiments were independently repeated three times. *p < 0.05.
Figure 4. Thiostrepton reduces mRNA and protein expressions of metastatic associated genes in NPC cells. A: Relative MMP-2, MMP-9, fascin-1, ezrin, and paxillin mRNA levels were determined by qRT-PCR. B: An ELISA assay was used to detect protein levels of MMP-2 and MMP-9. C: Fascin-1, ezrin, and paxillin protein levels were determined by Western blot. Experiments were independently repeated three times. *p < 0.05.
