Influence of the coating formulation on enzymatic digestibility and drug release from 5-aminosalicylic acid pellets coated with mixtures of high-amylose starch and Surelease® intended for colon-specific drug delivery

Abstract

Background: Colon-specific delivery of drugs can be achieved with dosage forms coated with biopolymers that are metabolized selectively by the colonic microflora and yet resistant to enzymatic digestion in the small intestine. Aim: The aim of this study was to study the influence of formulation factors on the performance of mixed films from high-amylose starches and Surelease®, applied using a spray-coating process, as potential colon-specific delivery devices. Methods: 5-Aminosalicylic acid-loaded pellets were prepared by an extrusion-spheronization process and film coated with mixtures of the starches and Surelease®. Optimization of the coating formulation, that is, starch-to-Surelease® ratio, film-coating thickness, and type of starch, was undertaken first in enzyme-free media resembling the conditions in the stomach and small intestine. The effect of curing of the film coating on the drug release profile upon storage was also evaluated. Optimized coating formulations were further assessed for enzymatic digestibility using artificial gastric and intestinal juices containing commercially available pepsin and pancreatin or α-amylase from hog pancreas, respectively. Finally, drug release was assessed in fluid-simulating conditions in the colon (SCF) containing Bacillus licheniformis α-amylase. Results: Film coatings comprising high-amylose starches and Surelease® in a ratio of 1:2 (w/w) and film thickness of approximately 45 μm were able to withstand the chemical and enzymatic environment of the upper gastrointestinal tract, in particular, resisted degradation by the pancreatic α-amylases. Stability of the coatings during storage was achieved with additional curing. In SCF, these coatings were susceptible to enzymatic degradation. Conclusions: This study showed that high amylose starch-mixed films can be successfully used as colon-specific delivery devices. The preparation of the coating dispersions described is simple and rapid, without the need to extract the amylose component of starch.

Keywords:

• α-Amylases
• colon-specific delivery
• film coating
• high-amylose starch
• in vitro drug release
• pellets

Acknowledgments

The authors thank the Fundação para a Ciência e Tecnologia, Portugal, for their financial support (SFR/BD/18330/2004).

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.