Differential Expression and Function of Peroxiredoxin 1 and Peroxiredoxin 6 in Cancerous MCF-7 and Noncancerous MCF-10A Breast Epithelial Cells

Abstract

Peroxiredoxins are thiol-specific antioxidant proteins whose expression is elevated in several cancers. We compared the expression and function of Prdx1 and Prdx6 between the MCF-7 mammary adenocarcinoma cell line and the noncancerous MCF-10A cell line. We found elevated Prdx1 expression in MCF-7 cells and comparable expression of Prdx6. Suppression of Prdx1 and/or Prdx6 resulted in a modest increase in peroxide-induced cytotoxicity of MCF-7 cells, and a dramatic increase in MCF-10A cytotoxicity with and without hydrogen peroxide treatment. Our data confirm a cytoprotective role for peroxiredoxins and suggest a synergistic role for Prdx1 and Prdx6 in MCF-10A cells.

Keywords: :

• Breast cancers
• Gene expression
• Molecular biology

ACKNOWLEDGMENTS

This research was supported by a National Cancer Institute grant (NIH-1R15CA13374D-01A1), and the Fairfield University Elizabeth DeCamp McInerney Chair of Health Science award to the PI.