Secreted Clusterin in Colon Tumor Cell Models and Its Potentialas Diagnostic Marker for Colorectal Cancer

Abstract

We studied the specific changes of the secreted protein clusterin and its cytoplasmic precursor regarding colorectal tumorigenesis, using in vitro differentiation of Caco-2 cells. In tumor-like stage, we observed an overexpression of both precursor and secreted clusterin, corroborated in the cell line SW-480. Noticeably, SW-620 cells (from a tumoral node, thus with metastatic capacity) did not show overexpression of either precursor or secreted clusterin, suggesting a downregulation related to local metastasis. We further investigated clusterin in serum, finding a significant increase in colorectal cancer patients, with 81% sensitivity, 79% specificity, and an area under the ROC curve of 0.85.

Keywords: :

• Clusterin
• Colorectal cancer
• Caco-2
• SW-480
• SW-620

ACKNOWLEDGMENTS

The authors thank ‘‘Complejo Hospitalario Universitario de Vigo,’’ in particular Concepción Fiaño, and ‘‘Centro de Transfusión de Galicia’’ for providing samples.