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Abstract
This study investigated the IGF-1-influence on oncological relevant genes in pleomorphic adenomas.
Therefore A64-tumorcells were stimulated by recombinant IGF-1. After RNA-extraction, transcript levels of hBD-1, hBD-2, hBD-3, DEFA1/3, DEFA4, S100A4, Psoriasin, DOC-1, EGF, EGFR, and IGFR were analyzed by qRT-PCR at t = 0, 4, 8, 24, 48, and 72 hr. The gene-products were visualized by immunostaining.
A64-tumor-cells were deficient for hBD-1 and IGF-1. IGF-1 downregulates hBD-2 and hBD-3 without influencing hBD-1-expression. IGF-1 only slightly affects DEFA1/3-, DEFA4-, S100A4-, Psoriasin-, DOC-1-, EGF-, EGFR-, and IGFR-gene-expression.
IGF-1-deficiency combined with low basic hBD-2-gene-expression and hBD-3-gene-expression might counteract, whereas hBD-1-deficiency promotes malignant transformation in pleomorphic adenomas.
Keywords: :
ACKNOWLEDGMENT
This study was supported by the BONFOR research foundation of the Medical Faculty of the University of Bonn. J. Winter (TP 10), M. Wenghoefer (TP 10), S. Jepsen (TP 2), JP. Allam (TP 1), N. Novak (TP 1), and W. Götz (TP 7) were supported by Deutsche Forschungsgemeinschaft (KFO 208).
DECLARATION OF INTERESTS
The authors declare that they have no competing interests.
| ABBREVIATIONS | ||
| DMEM | = | Dulbecco's Modified Eagle's Medium |
| Doc-1 | = | Deleted in oral cancer-1 |
| EGF(-R) | = | Epidermal growth factor (receptor) |
| HER-2 | = | Human epidermal growth factor receptor 2 |
| hBD | = | human beta defensin |
| IGF(-R) | = | Insulin-like growth factor (receptor) |
| n.d. | = | not detectable |
| OSCC | = | Oral squamous cell carcinoma |
| qRT-PCR | = | Quantitative realtime polymerase chain reaction |
| Rel. | = | Relative |
| SD | = | Standard deviation |