ABSTRACT
Purpose of the study: To explore the relationship between IL-1β expression and two common autoimmune thyroid diseases: Hashimoto thyroiditis (HT) and Graves’ disease (GD). Materials and methods: qRT-PCR, Quantiglo ELISA, and flow cytometry were used to evaluate the expression levels of IL-1β in serum, peripheral blood mononuclear cells (PBMCs), and thyroid tissue samples from patients with HT or GD. Local infiltration of monocytes was assessed by immunohistochemical study of patients’ thyroid tissue samples. Results: Although no significant differences in IL-1β levels were found between samples of serum from patients with HT or GD and normal controls, we found that IL-1β mRNA and protein levels in PBMCs of HT patients were significantly higher than those of patients with GD, which were in turn higher than the level in normal controls. In addition, IL-1β mRNA was also increased in thyroid gland tissue from patients with HT compared to those with GD, and this was accompanied by increased local infiltration of monocytes into thyroid tissues. Correlation analysis of the clinical samples validated the association of high IL-1β levels with the pathogenesis of HT. Conclusion: Our study suggests that IL-1β may be an active etiologic factor in the pathogenesis of HT and thus present a new target for novel diagnostics and treatment.
Acknowledgments
A special note of gratitude is expressed to Prof. Huaidong Song in Ruijin Hospital, Shanghai Jiaotong University, for providing the thyroid tissue-derived cDNAs of HT and GD patients.
We gratefully thank Prof. Wei Wei and Dr. Yujing Wu for their kind help with the FACS analysis performed at the Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine of the Education Ministry of China.
Funding
The research was financially supported by the National Natural Science Foundation of China (81270864) and national natural science foundation for fostering young scholars of China (by the First Affiliated Hospital of Anhui Medical University, grant No. 2013KJ16).
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. Potential conflicts of interest associated with this research.
Supplemental material
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