Effect of Non-cell Corynebacterium Parvum on Differentiation and Maturation of Bone Marrow-derived Dendritic Cells

Abstract

Corynebacterium parvum (CP), with their potent anti-tumor activities, has been well documented. Non-cell Corynebacterium Parvum (NCPP) is a neotype of biological preparation, which based on manipulating CP with nanotechnology. The present study was designed to investigate the effect of NCPP/CP on bone marrow derived dendritic cells (BMDCs) in tumor-bearing mice, especially focused on the differentiation and maturation of these BMDCs. BM cells from tumor-bearing mice administrated with NCPP/CP were analyzed by flow cytometry, which exhibit enhanced numbers of DCs and macrophages. In the meanwhile, flow cytometry analysis showed mild but significant difference for CD80 expression on these LPS- treated BMDCs between NCPP/CP administrated mice and the control animals. Furthermore, antigen presenting assay for these LPS-treated BMDCs showed significant difference for cytolytic assay of CD8+T cells against B16 melanoma cells, which indicate that NCPP treatments have enhanced the cytolytic rates of CD8+T cells from 47.9%±2.3% to 54.2%±2.4%. The data suggest that NCPP/CP treatment can efficiently facilitate the generation of BMDCs in vivo and enhance the maturation of these BMDCs in vitro.

• Corynebacterium parvum
• NCPP
• Dendritic cells
• CD8+T cells
• Antigen presentation

ACKNOWLEDGMENTS

This work was supported by the Natural Science Foundation of China (Grant No. 81072407) and the Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20110131120055) and Natural Science Foundation of Shandong Province (Grant No. #ZR2011HQ031).

Declaration of Interest:The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.