Apoptosis of Peripheral CD4⁺ T-Lymphocytes in End-Stage Renal Disease Patients Under Hemodialysis and rhEPO Therapies

Figures & data

Table 1. Blood cell count and T-cell phenotype in controls and HD patients

	Controls (n = 11)	HD patients (n = 47)
Hb (g/dL)	15.02 ± 1.48	11.56 ± 1.72
White blood cells (×10^9/L)	6.42 ± 1.50	6.78 ± 2.14
Lymphocytes (×10^9/L)	1.91 ± 0.341	1.74 ± 0.833
CD3^+ T cells (×10^9/L)	1.46 ± 0.268	1.21 ± 0.607
CD4^+ T cells (×10^9/L)	0.941 ± 0.216	0.747 ± 0.410
CD8^+ T cells (×10^9/L)	0.523 ± 0.202	0.484 ± 0.325
CD4/CD8 ratio	1.96 (1.54–2.48)	1.84 (1.18–2.24)
Neutrophils (×10^9/L)	4.06 ± 1.41	4.31 ± 1.68
Monocytes (×10^9/L)	0.237 ± 0.072	0.488 ± 0.181
Eosinophils (×10^9/L)	0.173 ± 0.07	0.199 ± 0.144
Neutrophil/lymphocyte ratio	1.90 (1.74–2.84)	2.4 (1.71–3.86)

Note: *p < 0.05 versus controls.

Figure 1. Apptosis evaluation by Annexin-V assay, in a group of patients and controls (A) and in a group of patients before and after the HD procedure (B). Two different CD3+/CD4+ T-cells populations were classified according to the differents staining of Annexin-V and 7-AAD: cells underdoing apoptosis (Annexin-V⁺/7-AAD⁻) and cells in the latest apoptosis stage or death (Annexin-V⁺/7-AAD⁺). a) P < 0.05; b) p < 0.01; HD = hemodialyse

Figure 2. Correlations between Hemodialysis time with the proportion of (A) CD4+ and (B) CD3V+ T cells that died or are in the latest stage of apoptosis.

Table 2. Lymphocyte count and T-cell phenotype in HD patients, before and immediately after HD procedure

	HD patients (n = 12)
	Before HD	After HD
White blood cells (×10^9/L)	6.11 ± 1.66	5.74 ± 1.44
Lymphocytes (×10^9/L)	1.76 ± 0.786	1.55 ± 0.549
CD3^+ T cells (×10^9/L)	1.46 ± 0.627	1.40 ± 0.477
CD4^+ T cells (×10^9/L)	0.860 ± 0.384	0.950 ± 0.364
Neutrophils (×10^9/L)	3.64 ± 1.15	3.56 ± 1.17
Monocytes (×10^9/L)	0.519 ± 0.145	0.446 ± 0.157
Eosinophils (×10^9/L)	0.169 ± 0.113	0.128 ± 0.091
Neutrophil/lymphocyte ratio	2.52 ± 1.46	2.75 ± 1.76

Note: *p < 0.05 versus before HD.

Girndt M, Sester M, Sester U, Kaul H, Köhler H. Molecular aspects of T and B cell function in uremia. Kidney Int. 2001;59:206–211.

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