Figures & data
Figure 1. Effect of PC on renal function. The levels of serum BUN and creatinine at 72 h after cisplatin injection (C, control cells; PC, PC only-treated mice; CIS, cisplatin-treated cells; Cis + PC, PC with cisplatin-treated mice).
Note: *p < 0.05 versus C, **p < 0.05 versus CIS.
Figure 2. Effects of PC on renal histology (original magnification 100×, 400×). (A) Changes in renal histology 72 h after cisplatin-induced renal injury. The vehicle with cisplatin-treated kidneys showed marked injury, with sloughing of tubular epithelial cells and loss of the brush border. These changes were reduced by PC treatment. (B) Percentage of damaged tubules.
Note: *p < 0.05 versus C, **p < 0.05 versus CIS.
Figure 3. (A, B) Densitometric analysis of Bax and Bcl-2 immunoblots. PC treatment recovered the expression of Bcl-2 protein which was decreased by cisplatin treatment. (C) Representative photomicrographs of Bax and Bcl-2 immunostaining. (D) Quantitative analysis of TUNEL-positive cells. PC treatment reduced TUNEL-positive cells. (E) Colorimetric analysis of caspase-3 activity. PC treatment suppressed caspase-3 activity in kidneys at 72 h after cisplatin treatment.
Note: *p < 0.05 versus C, **p < 0.05 versus CIS.
Figure 4. (A) Effect of PC on cytotoxicity and viability in HK-2 cells. (A) LDH leakage was increased in vehicle with cisplatin-treated HK-2 cells. PC treatment reduced LDH leakage in cisplatin-treated HK-2 cells. (B) Viable cells were reduced by cisplatin treatment. PC treatment increased the survival of cisplatin-treated HK-2 cells. (C) Number of FLICA-positive cells, apoptotic cells. PC treatment reduced FLICA-positive cells. (D) Representative photomicrograph of FLICA staining results (original magnification 400×).
Note: *p < 0.05 versus C, **p < 0.05 versus CIS.
Figure 5. (A–C) Densitometric analysis of Bax, Bcl-2, and caspase-9 immunoblots. PC treatment decreased the expression of Bax and caspase-9 protein in HK-2 cells at 24 h after cisplatin treatment. PC treatment recovered the expression of Bcl-2 protein which was decreased by cisplatin treatment. (D) Representative photomicrographs of Bax, Bcl-2, and caspase-9 immunostaining. (E) Colorimetric analysis of caspase-3 activity. PC treatment suppressed caspase-3 activity in cisplatin-treated cells at 24 h after cisplatin treatment.
Note: *p < 0.05 versus C, **p < 0.05 versus CIS.
Figure 6. Representative photomicrographs of immunostaining and densitometric analysis for p-p38, p-JNK, and p-ERK. PC treatment decreased phosphorylation of p-p38, p-JNK, and p-ERK in HK-2 cells at 24 h after cisplatin treatment.
Note: *p < 0.05 versus C, **p < 0.05 versus CIS.
