Protective Effect of Infliximab on Ischemia/Reperfusion-Induced Damage in Rat Kidney

Figures & data

Table 1. Effects of infliximab on MDA, MPO, GSH and SOD levels in rats exposed to renal I/R.

	MDA	MPO	GSH	SOD
	(nmol/mg protein)	(U/mg protein)	(nmol/mg protein)	(U/mg protein)
Sham	0.52 ± 0.05	0.47 ± 0.07	24 ± 2.56	15.18 ± 1.76
I/R	1.33 ± 0.16^a	4.30 ± 0.39^a	12.56 ± 0.73^b	8.59 ± 0.69^b
I/R + infliximab (bi)	0.53 ± 0.11^c	1.5 ± 0.16^c	18.25 ± 1.33^d	12.02 ± 1.10^d
I/R+ infliximab (br)	0.55 ± 0.03^c	3.22 ± 0.30^c,e	17.95 ± 1.57^d	10.70 ± 0.91^d

Notes: GSH, glutathione; I/R, ischemia–reperfusion; MDA, malondialdehyde; MPO, myeloperoxidase; SOD, superoxide dismutase. Values are expressed as the mean ± SEM.

(bi): before ischemia, (br): before reperfusion.

^a,bp < 0.05 versus Sham group.

^c,d p < 0.05 versus I/R group.

^ep < 0.05 versus I/R + infliximab (bi) group.

Table 2. The comparison of severity of renal damage among groups.

Groups	Renal damage
Sham	0.5 ± 0.3
I/R	14.3 ± 0.6^a
I/R + infliximab (bi)	7.8 ± 0.3^a,b
I/R+ infliximab (br)	8.14 ± 0.5^a,b,c

Notes: (bi): before ischemia, (br): before reperfusion.

^aMean value was significantly increased compared with that of the Sham group (p < 0.05).

^bMean value was significantly decreased compared with that of the I/R group (p < 0.05)

^cNot significant when compared with group I/R + infliximab (bi) (p > 0.05).

Figure 1. (A) Sham; renal tubules and glomeruli are intact. H&E ×66. (B) Sham; appearance of brush border (arrows). PAS ×132. (C) I/R group; tubules show diffuse and marked necrosis (*). H&E ×66. (D) I/R group; notice lost of the brush border and glomerular changes. PAS ×132. (E) I/R + infliximab (bi); despite tubular degeneration are visible in some area, glomeruli are seen intact. H&E ×66. (F) I/R + infliximab (bi); the loss of brush border was marked in affected tubules. PAS ×132. (G) I/R+ infliximab (br); moderate tubular damage and interstitial congestion are observed. H&E ×66. (H) I/R+ infliximab (br); the view of brush border is similar to ischemia group. PAS ×132.