The effect of simvastatin and erythropoietin on renal fibrosis in rats with unilateral ureteral obstruction

Figures & data

Figure 1. Representative photomicrographs (×40 magnifications) of histochemical staining for Masson’s trichrome demonstrating collagen accumulation in the obstructed rat kidneys. Immunostaining for TGF-β, NFκB, PDGF-B, bFGF and fibronectin are seen in tubular cytoplasms, in addition NFκB staining seen in tubular nuclei and fibronectin staining also seen in interstitium. All staining is the densest in group control and faint in group sham.

Figure 2. The effect of Epo and Simv on TGF-β Expression. *p < 0.01 group sham versus group Epo, group Simv and group control, **p < 0.01 group control versus group Epo and group Simv.

Figure 3. The effect of Epo and Simv on NFκB expression. *p < 0.01 group sham versus group Epo, group Simv and group control, **p  <  0.01 group control versus group Epo.

Figure 4. The effect of Epo and Simv on expression of PDGF-B. *p <  0.01 group sham versus group Epo, group Simv and group control.

Figure 5. The effect of Epo and Simv on expression of bFGF. *p <  0.01 group sham versus group Epo, group Simv and group control, **p  <  0.01 group control versus group Epo.

Figure 6. The effect of Epo and Simv on expression of fibronectin. *p <  0.01 group sham versus group Epo, group Simv and group control. **p <  0.01 group control versus group Epo and group Simv. ***p <  0.01 group Epo versus group Simv.

Figure 7. The effect of Epo and Simv on Masson’s trichrome staining. *p <  0.01 group sham versus group Epo, group Simv and group control, **p <  0.01 group control versus group Epo and group Simv.

Table 1. Combined score of TGF-β, NFκB, PDGF B, bFGF and positive staining area % of fibronectin and Masson’s trichrome.

	Group Sham median (min–max)	Group Epo median (min–max)	Group Simv median (min–max)	Group control median (min–max)	p
TGF-β	3.00 (2.00–3.00)	5.00 (4.00–7.00)	5.00 (4.00–6.00)	7.00 (6.00–8.00)	<0.01*
NFκB	3.00 (3.00–5.00)	4.00 (3.00–6.00)	5.00 (4.00–8.00)	6.00 (5.00–6.00)	<0.01‡
PDGF-B	2.50 (2.00–3.00)	6.00 (4.00–8.00)	6.00 (4.00–6.00)	6.00 (4.00–6.00)	<0.01†
bFGF	2.00 (2.00–3.00)	5.00 (3.00–6.00)	6.00 (4.00–6.00)	6.00 (4.00–7.00)	<0.01§
Fibronectin	5.35 (1.10–9.50)	11.30 (6.20–25.70)	20.25 (8.70–35.40)	29.85 (16.30–83.40)	<0.01¥
Masson’s trichrome	4.40 (2.20–8.40)	19.75 (10.70–35.70)	24.40 (15.80–38.10)	42.05 (21.40–62.80)	<0.01£

Notes: *Group sham versus group Epo, group Simv and group control, *group control versus group Epo and group Simv.

‡Group sham versus group Epo, group Simv and group control, ‡group control versus group Epo.

†Group sham versus group Epo, group Simv and group control.

§Group sham versus group Epo, group Simv and group control, §group control versus group Epo.

¥Group sham versus group Epo, group Simv and group control, ¥group control versus group Epo and group Simv, ¥ group Epo versus group Simv.

£Group sham versus group Epo, group Simv and group control, £group control versus group Epo and group Simv.