Figures & data
Figure 1. Effect of ozone OP on apoptosis of NRK-52E cells subjected to H/R injury. The apoptosis was examined using annexin V-FITC/PI staining and flow cytometry analysis. The experiment was repeated three times and representative data are shown.
![Figure 1. Effect of ozone OP on apoptosis of NRK-52E cells subjected to H/R injury. The apoptosis was examined using annexin V-FITC/PI staining and flow cytometry analysis. The experiment was repeated three times and representative data are shown.](/cms/asset/3cc76e5e-84fd-4eb7-aa90-b4eb0bd998f9/irnf_a_950934_f0001_b.jpg)
Figure 2. Staining with Hoechst 33258 and immunocytochemistry. (A and D) cells from control group, (B and E) cells from H/R group, (C and F) cells from ozone 40 preconditioning group. (A–C) Hoechst 33258 staining. (D–F) cleaved caspase-3 immunohistochemical staining. All Hoechst 33258 and immunohistochemical staining, original magnification ×200.
![Figure 2. Staining with Hoechst 33258 and immunocytochemistry. (A and D) cells from control group, (B and E) cells from H/R group, (C and F) cells from ozone 40 preconditioning group. (A–C) Hoechst 33258 staining. (D–F) cleaved caspase-3 immunohistochemical staining. All Hoechst 33258 and immunohistochemical staining, original magnification ×200.](/cms/asset/2ae7b690-3136-4dff-8dc9-e5d95f6c4d7d/irnf_a_950934_f0002_b.jpg)
Figure 3. (A) Assay of SOD activity in NRK-52E cells of different groups. (B) Assay of MDA content in NRK-52E cells of different groups. (C) Assay of LDH leaking ratio in NRK-52E cells of different groups. Bars represent means ± SEM (n = 4); *p < 0.05 versus Group C, #p < 0.05 versus Group H/R.
![Figure 3. (A) Assay of SOD activity in NRK-52E cells of different groups. (B) Assay of MDA content in NRK-52E cells of different groups. (C) Assay of LDH leaking ratio in NRK-52E cells of different groups. Bars represent means ± SEM (n = 4); *p < 0.05 versus Group C, #p < 0.05 versus Group H/R.](/cms/asset/f86c6de0-6247-433d-8350-e243a4ac22d6/irnf_a_950934_f0003_b.jpg)
Figure 4. Representative Western blots showed the effects of ozone on PARP-1, Bcl-2 and Bax expression in NRK-52E cells of different groups. (A) Representative Western blots showing the effects of ozone preconditioning on PARP-1, Bcl-2 and Bax expression. (B) Relative band densities of PARP-1 to the mean value of the control. (C) Relative band densities of Bcl-2 to the mean value of the control. (D) Relative band densities of Bax to the mean value of the control (means ± SEM; *p < 0.05 vs. Group C; #p < 0.05 vs. Group H/R 3 h; •p < 0.05 vs. Group H/R 6 h; ▪p < 0.05 vs. Group H/R 12 h; ★p < 0.05 vs. Group H/R 24 h).
![Figure 4. Representative Western blots showed the effects of ozone on PARP-1, Bcl-2 and Bax expression in NRK-52E cells of different groups. (A) Representative Western blots showing the effects of ozone preconditioning on PARP-1, Bcl-2 and Bax expression. (B) Relative band densities of PARP-1 to the mean value of the control. (C) Relative band densities of Bcl-2 to the mean value of the control. (D) Relative band densities of Bax to the mean value of the control (means ± SEM; *p < 0.05 vs. Group C; #p < 0.05 vs. Group H/R 3 h; •p < 0.05 vs. Group H/R 6 h; ▪p < 0.05 vs. Group H/R 12 h; ★p < 0.05 vs. Group H/R 24 h).](/cms/asset/18d0802a-d789-48cb-b4dc-90c3cc5a8e50/irnf_a_950934_f0004_b.jpg)