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Renal Failure Volume 37, 2015 - Issue 6
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Laboratory Study

Effect of triptolide on expression of thrombospondin-1 and transforming growth factor-β1 in renal tubular cells

Rong ZengEvidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, China, ;Key Laboratory of Clinical Translational Research and Evidence-Based Medicine Center of Gansu Province, Lanzhou, Gansu, China, ;The Second Clinical Medicine College of Lanzhou University, Lanzhou, Gansu, China, and
,
Lei DuanEvidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, China, ;Key Laboratory of Clinical Translational Research and Evidence-Based Medicine Center of Gansu Province, Lanzhou, Gansu, China, ;The Second Clinical Medicine College of Lanzhou University, Lanzhou, Gansu, China, and
,
Lina SunThe First Clinical Medicine College of Lanzhou University, Lanzhou, Gansu, China
,
Yuke KongThe Second Clinical Medicine College of Lanzhou University, Lanzhou, Gansu, China, and
&
Kehu YangEvidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, China, ;Key Laboratory of Clinical Translational Research and Evidence-Based Medicine Center of Gansu Province, Lanzhou, Gansu, China, Correspondence[email protected]
Pages 1039-1043 | Received 24 Dec 2014, Accepted 18 Mar 2015, Published online: 06 May 2015

Figures & data

Figure 1. Test flow chart.

Figure 1. Test flow chart.

Figure 2. Effects of triptolide on expression of TSP1 and TGF-β mRNA in AngII-induced HK-2 cells (Real Time PCR). Note: Blank control (no any drugs were added); positive control (10−7 mol/L AngII only); R1: triptolide 0.1 µg/L + 10−7 mol/L AngII; R2: triptolide 1 µg/L + 10−7 mol/L AngII; R3: triptolide 10 µg/L + 10−7 mol/L AngII.

Figure 2. Effects of triptolide on expression of TSP1 and TGF-β mRNA in AngII-induced HK-2 cells (Real Time PCR). Note: Blank control (no any drugs were added); positive control (10−7 mol/L AngII only); R1: triptolide 0.1 µg/L + 10−7 mol/L AngII; R2: triptolide 1 µg/L + 10−7 mol/L AngII; R3: triptolide 10 µg/L + 10−7 mol/L AngII.

Figure 3. Effects of triptolide on expression of TSP1 and TGF-β protein in AngII-induced HK-2 cells. Note: Blank control (no any drugs were added); positive control (10−7 mol/L AngII only); R1: triptolide 0.1 µg/L + 10−7 mol/L AngII; R2: triptolide 1 µg/L + 10−7 mol/L AngII; R3: triptolide 10 µg/L + 10−7 mol/L AngII.

Figure 3. Effects of triptolide on expression of TSP1 and TGF-β protein in AngII-induced HK-2 cells. Note: Blank control (no any drugs were added); positive control (10−7 mol/L AngII only); R1: triptolide 0.1 µg/L + 10−7 mol/L AngII; R2: triptolide 1 µg/L + 10−7 mol/L AngII; R3: triptolide 10 µg/L + 10−7 mol/L AngII.

Figure 4. Effects of triptolide on expression of TSP1 and TGF-β protein in AngII-induced HK-2 cells. Note: Blank control (no any drugs were added); positive control (10−7 mol/L AngII only); R1: triptolide 0.1 µg/L + 10−7 mol/L AngII; R2: triptolide 1 µg/L + 10−7 mol/L AngII; R3: triptolide 10 µg/L + 10−7 mol/L AngII.

Figure 4. Effects of triptolide on expression of TSP1 and TGF-β protein in AngII-induced HK-2 cells. Note: Blank control (no any drugs were added); positive control (10−7 mol/L AngII only); R1: triptolide 0.1 µg/L + 10−7 mol/L AngII; R2: triptolide 1 µg/L + 10−7 mol/L AngII; R3: triptolide 10 µg/L + 10−7 mol/L AngII.

Figure 5. Effects of different concentration triptolide on levels of total and active TGF-β. Note: Blank control (no any drugs were added); positive control (10−7 mol/L AngII only); R1: triptolide 0.1 µg/L + 10−7 mol/L AngII; R2: triptolide 1 µg/L + 10−7 mol/L AngII; R3: triptolide 10 µg/L + 10−7 mol/L AngII.

Figure 5. Effects of different concentration triptolide on levels of total and active TGF-β. Note: Blank control (no any drugs were added); positive control (10−7 mol/L AngII only); R1: triptolide 0.1 µg/L + 10−7 mol/L AngII; R2: triptolide 1 µg/L + 10−7 mol/L AngII; R3: triptolide 10 µg/L + 10−7 mol/L AngII.

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