Figures & data
Figure 1. (A) The morphology of rabbit BM-MSCs, Scale bar, 50 μm. (B) The phenotypes of BM-MSCs analysis by FCM at third passages. FCM analysis revealed that BM-MSCs were positive for CD44 and CD71 and negative for CD34 and CD45.
![Figure 1. (A) The morphology of rabbit BM-MSCs, Scale bar, 50 μm. (B) The phenotypes of BM-MSCs analysis by FCM at third passages. FCM analysis revealed that BM-MSCs were positive for CD44 and CD71 and negative for CD34 and CD45.](/cms/asset/12cc5b51-3df3-4eb5-b05a-2e068a63d941/irnf_a_1073542_f0001_c.jpg)
Figure 2. Prussian blue staining of SPIO labeled BM-MSCs, and the efficiency is up to 100%. Note: Scale bar, 50 μm.
![Figure 2. Prussian blue staining of SPIO labeled BM-MSCs, and the efficiency is up to 100%. Note: Scale bar, 50 μm.](/cms/asset/cfe8b741-cc7c-46ce-9eaf-bf2039c08c4b/irnf_a_1073542_f0002_c.jpg)
Table 1. The average viability of SPIO-MSCs by Trypan blue staining test.
Figure 3. Detectable time point of labeled cells on MRI in vitro (T2*WI). Notes: An increasing signal intensity of labeled cells is suspended in gelatin. Number of labeled cells are suspended in 50 L gelatin. On the T2*WI, there is a significantly lower signal intensity in 5 × 104, cells/mL (A) and 5 × 105 cells/mL SPIO-MSCs compared with unlabeled counterparts, but the signal intensity of week 3 with 5 × 104 cells/mL and week 4 with 5 × 105 cells/mL is almost the same as that of control group.
![Figure 3. Detectable time point of labeled cells on MRI in vitro (T2*WI). Notes: An increasing signal intensity of labeled cells is suspended in gelatin. Number of labeled cells are suspended in 50 L gelatin. On the T2*WI, there is a significantly lower signal intensity in 5 × 104, cells/mL (A) and 5 × 105 cells/mL SPIO-MSCs compared with unlabeled counterparts, but the signal intensity of week 3 with 5 × 104 cells/mL and week 4 with 5 × 105 cells/mL is almost the same as that of control group.](/cms/asset/66de17f0-000d-42bb-90b1-f2251c2d1ca5/irnf_a_1073542_f0003_b.jpg)
Figure 4. Histological detection of labeled SPIO-MSCs in kidneys. Prussian blue and H&E stains of kidney after AKI and application of labeled SPIO-MSCs, all pictures showed a predominant presence of cells in the renal medulla corresponding to the regions of signal loss in MRI. The regions of signal intensity in MRI is the lowest at 72 h after SPIO-MSCs administration. The kidneys show more uptake of iron oxide particles in the renal medulla cells caused by uptake of circulating iron oxide particles, the Scale bar, 50 μm.
![Figure 4. Histological detection of labeled SPIO-MSCs in kidneys. Prussian blue and H&E stains of kidney after AKI and application of labeled SPIO-MSCs, all pictures showed a predominant presence of cells in the renal medulla corresponding to the regions of signal loss in MRI. The regions of signal intensity in MRI is the lowest at 72 h after SPIO-MSCs administration. The kidneys show more uptake of iron oxide particles in the renal medulla cells caused by uptake of circulating iron oxide particles, the Scale bar, 50 μm.](/cms/asset/d4a64119-31ed-49bf-95dd-ecdfb4c92dcf/irnf_a_1073542_f0004_c.jpg)