Figures & data
Figure 1. Renal proximal tubular epithelial cells were treated Methotrexate (1, 10 and 100 μM, n = 6), assessed by MTT. ***p < 0.001 versus vehicles.
Figure 2. Renal proximal tubular epithelial cells were treated with 17β-estradiol (100 nm, n = 6), GPER-1 agonist G-1 (100 nm, n = 6), ERα agonist PPT (100 nm, n = 6), ERβ agonist DPN (100 nm, n = 6) for 30 min prior to treatment with methotrexate (10 μM, n = 6) for 48 h. Cell viability was determined by MTT assay. ***p < 0.001 versus vehicles, ++p < 0.01, +++p < 0.001 versus MTX.
Figure 3. (a) IL-1β and (b) IL-6 levels in renal proximal tubular epithelial cells. ***p < 0.001 versus vehicles, +p < 0.01, ++p < 0.01, +++p < 0.001 versus MTX.
Figure 4. (a) SOD and (b) MDA activities in renal proximal tubular epithelial cells. ***p < 0.001 versus vehicles, +p < 0.01, ++p < 0.01 versus MTX.
