Figures & data
Figure 1. Effects of IPA3 on platelet aggregation and phosphorylation of proteins. (A) Platelet aggregation was measured by using aspirin-treated washed human platelets (2 × 108 cells/ml). Samples were pretreated with varying concentrations of IPA3 for 5 min at 37 °C and activated with (A.1) 250 µM AYPGKF or (A.2) 100 ng/ml convulxin at 37 °C while stirring. (B) Platelets were incubated with 10 µM IPA3 for 5 min and reaction was stopped by 6.6 N perchloric acid. Phosphorylation status of threonine (B.1), tyrosine (B.2) and PKC substrate residues (B.3) were analyzed by Western blot analysis and probed with phospho-specific antibodies, respectively. (C) Phosphorylation of Serine residue 473 on Akt, tyrosine residues 525/526 on Syk, tyrosine residue 759 on PLCγ2, and tyrosine 416 on Src family kinases were analyzed by Western blot analysis using phospho-specific antibodies, in platelets incubated with 10 µM IPA3.
