Chromosome aberrations determined by sFISH and G-banding in lymphocytes from workers with internal deposits of plutonium

Figures & data

Table 1. Individual data on date of birth, smoking status, analysis technique, sampling times and red bone marrow doses from internal plutonium α-particle irradiation and external γ-irradiation.

			Red bone marrow dose from: internal plutonium α-particles (mGy), and external γ-rays (mGy)
Sample ID	Date of birth	Smoking status	Group 1	Group 2	Group 3	Group 4	Group 5
			G-band	G-band	G-band	sFISH	sFISH
			1978–1982	1986–1991	1993–1994	1996–2003	2004–2008
1	04/09/1924	S	19.2
			145.6
2	12/01/1923	S	10.7
			825.4
3	21/04/1921	S	33.2
			289.7
4	11/11/1919	S	12.8
			240.6
5	11/04/1921	N	41.0
			453.8
6	28/06/1927	S	17.2
			418.6
7	25/11/1924	N/S	13.2	16.3
			232.1	303.7
8	26/01/1942	S	14.8	23.3	27.0
			332.7	342.2	344.1
9	16/08/1929	N/N/S	15.6	24.0	27.7
			472.7	472.7	472.7
10	18/05/1929	S	43.1	50.8	54.3	57.7
			112.6	112.6	112.6	112.6
11	05/03/1935	N	15.3	24.8		32.1	36.0
			317.2	322.8		325.1	325.1
12	19/11/1928	S		11.9
				547.9
13	18/12/1928	S		11.70
				133.2
14	23/12/1936	S		10.4		13.6
				37.0		37.0
15	16/12/1932	S			11.0
					211.0
16	17/03/1921	S				14.8
						225.0
17	08/04/1941	S				11.4
						594.1
18	25/07/1918	S				16.2
						236.0
19	09/06/1928	S				12.7
						649.6
20	16/11/1919	S				25.2
						197.6
21	19/06/1925	S				22.1
						304.5
22	30/03/1927	N				15.6
						684.2
23	21/04/1923	S				15.8
						740.0
24	26/06/1929	N				11.5
						1336.3
25	06/10/1929	S				38.1
						209.3
26	29/06/1926	S				10.1
						726.4
27	03/06/1929	S				10.1
						486.5
28	19/04/1936	S				13.3
						745.1
29	07/08/1928	S				11.6
						775.8
30	09/12/1924	S				14.2
						624.0
31	01/02/1924	S				24.1
						296.5
32	11/11/1926	S				15.6
						285.3
33	04/06/1925	S				22.8	24.7
						811.1	811.1
34	01/11/1930	S				40.7	46.5
						1077.1	1077.1
35	16/04/1927	S					12.7
							704.1
36	16/08/1929	S					35.6
							472.7
37	15/04/1931	S					17.3
							408.5
38	17/08/1950	S					10.3
							401.2

N: non-smoker; S: ever smoker.

Table 2. Data on age, smoking, red bone marrow dose and chromosome aberration frequencies assessed using sFISH.

	Group 4	Group 5	Combined
Number of individuals	22	7	29
Mean age (years) (range)	71 (57–81)	72 (56–79)	71 (56–81)
Number of ever smokers (%)	19 (86.4)	6 (85.7)	25 (86.2)
Mean internal α-particle dose (mGy) (range)	20.4 (10.0–57.7)	26.1 (10.3–46.5)	21.8 (10.0–57.7)
Mean external γ-ray dose (mGy) (range)	522 (37–1336)	600 (325–1077)	541 (37–1336)
Number of cells scored	26400	9528	35928
Number of genome equivalents	9105	3259	12364
Number of translocations	170	64	234
Translocations per genome equivalent ± SE × 10^−3	18.67 ± 1.43	19.64 ± 2.46	18.93 ± 1.24
Number of dicentrics	10	4	14
Dicentrics per genome equivalent ± SE × 10^−3	1.10 ± 0.35	1.23 ± 0.61	1.13 ± 0.30
Number of dicentric equivalents	2	7	9
Dicentrics + dicentric equivalents per genome equivalent ± SE × 10^−3	1.32 ± 0.38	3.38 ± 1.02	1.86 ± 0.39
Number of unstable complexes (rogue cells)	18 (1)	8 (1)	26 (2)
Unstable complex aberrations per total cells ± SE × 10^−3	0.68 ± 0.16	0.84 ± 0.30	0.72 ± 0.14
Number of stable complexes (insertions)	7 (6)*	6 (1)	13 (7)^a
Stable complex aberrations per total cells ± SE × 10^−3	0.27 ± 0.10	0.63 ± 0.26	0.36 ± 0.10

^aTwo insertions present in one cell as part of one complex aberration.

Table 3. Data on age, smoking, red bone marrow dose and simple chromosome aberration frequencies assessed using G-banding.

	Group 1	Group 2	Group 3	Combined
Number of individuals	11	8	4	23
Mean age (years) (range)	53 (38–61)	57 (47–63)	61 (52–64)	56 (38–64)
Number of ever smokers (%)	7 (63.6)	6 (75.0)	4 (100.0)	17 (73.9)
Mean internal α-particle dose (mGy) (range)	21.5 (10.7–43.1)	21.7 (10.4–50.8)	30.0 (11.0–54.3)	23.0 (10.4–54.3)
Mean external γ-ray dose (mGy) (range)	349 (113–825)	284 (37–548)	285 (113–473)	315 (37–825)
Number of cells scored	1100	800	400	2300
Number of translocations	10	13	3	26
Translocations per cell ± SE × 10^−3	9.09 ± 2.87	16.25 ± 4.51	7.50 ± 4.33	11.30 ± 2.22
Number of dicentrics	2	2	0	4
Dicentrics per cell ± SE × 10^−3	1.82 ± 1.29	2.50 ± 1.77	0	1.74 ± 0.87

Table 4. Comparison of complex aberrations determined by sFISH.

	Controls^a	External workers 50–499 mGv^a	External workers > 500 mGy^a	Pu workers
Number of individuals	97	247	115	29
Mean internal α-particle dose (mGy) (range)	0.4 (0–4.5)	1.8 (0–9.4)	3.3 (0–9.6)	21.8 (10.0–57.7)
Mean external γ-ray dose (mGy) (range)	22 (0–50)	267 (57–499)	687 (500–1563)	541 (37–1336)
Number of cells scored	132700	271709	131418	35928
Number of unstable complexes (rogue cells)	59 (16)	96 (12)	78 (25)	26 (2)
Unstable complex aberrations per total cells ± SE × 10^−3	0.44 ± 0.06	0.35 ± 0.04	0.59 ± 0.07	0.72 ± 0.14
Number of stable complexes (insertions)	47 (27)	59 (32)^b	60 (45)	13 (7)^b
Stable complex aberrations per total cells ± SE × 10^−3	0.35 ± 0.05	0.22 ± 0.03	0.46 ± 0.06	0.36 ± 0.10

^aData from Tawn et al. (2015b). ^bTwo insertions present in one cell as part of one complex aberration.

Tawn EJ, Curwen GB, Jonas P, Gillies M, Hodgson L, Cadwell KK. 2015b. Chromosome aberrations determined by FISH in radiation workers from the Sellafield Nuclear Facility. Radiat Res. 184:296–303.

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