Abstract
Hydrogen peroxide was produced from bran by a two-step process using cellulase/xylanase and glucose oxidase, sequentially. The decolourization efficiency of the produced reagent was tested using paprika oil dye (effluent from industrial source) and high levels of colour removal (96%) were achieved after saponification pre-treatment and hydrogen peroxide application. The method is economically and environmentally advantageous since lower energy and chemical input are needed and wastewater pollution is considerably reduced. At the same time, the utilization of waste materials was successfully achieved.
Acknowledgements
The authors would like to acknowledge the industrial source for the bran and dye supply.
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article. Carla Silva would like to acknowledge the Portuguese Fundação para a Ciência e a Tecnologia (FCT) for funding under the scholarship SFRH/BPD/46515/ 2008.