Figures & data
Figure 1 Effect of cold and immobilization alone or consecutively on NCX1 mRNA (left) and protein (right) levels in the kidney. UC—untreated control, 1DC—exposure to 4°C for 1 day, 7IMO1RT—daily exposure to immobilization for 7 days, 2 h per day with subsequent rest for 1 day at room temperature, 7IMO1DC—daily immobilization for 7 days, 2 h per day with subsequent exposure to cold for 1 day. Values are group mean ± SEM; n, 6–8 rats per group. One-way ANOVA: p = 1.191 × 10− 5, F = 27.3625 (left), p = 3.938 × 10− 5, F = 21.6354 (right). *p < 0.05 and **p < 0.01 vs. UC; ++p < 0.01 vs. 7IMO1RT.
Figure 2 Effect of cold and immobilization alone or consecutively on NCX1 activity in the kidney. UC—untreated control, 1DC—exposure to 4°C for 1 day, 7IMO1RT—daily exposure to immobilization for 7 days, 2 h per day with subsequent rest for 1 day at room temperature, 7IMO1DC—daily immobilization for 7 days, 2 h per day with subsequent exposure to cold for 1 day. Values are group mean ± SEM; n, 6–8 rats per group. One-way ANOVA: p = 4.943 × 10− 8, F = 74.7025. *p < 0.05 vs. UC.
Figure 3 Effect of cold and immobilization alone or consecutively on NCX1mRNA (left) and protein (right) levels. UC—untreated control, 7DC—exposure to 4°C for seven consecutive days, 7IMO7RT—daily exposure to immobilization for 7 days, 2 h per day with subsequent rest for 7 days at room temperature, 7IMO7DC—daily exposure to immobilization for 7 days, 2 h per day with subsequent exposure to cold for seven consecutive days. Values are displayed as mean ± SEM; n, 6–8 rats per group. One-way ANOVA: p = 8.425 × 10− 4, F = 11.244 (left), p = 4.662 10− 14, F = 793.3528 (right) **p < 0.01 vs. UC; +p < 0.05 vs. 7IMO7RT.
Figure 4 Effect of cold and immobilization alone or consecutively on NCX1 activity in the kidney. UC—untreated control, 7DC—exposure to 4°C for 7 days, 7IMO7RT—exposure to daily immobilization for 7 days, 2 h per day with subsequent rest for 7 days at room temperature, 7IMO7DC—daily immobilization for 7 days, 2 h per day with subsequent exposure to cold for 7 days. Values are group mean ± SEM; n, 6–8 rats per group. One-way ANOVA: p = 2.446 × 10− 9, F = 126.078. **p < 0.01 vs. UC.
Figure 5 Effect of long-term cold exposure, and a single immobilization alone or after long-term cold on NCX1 mRNA levels in the kidney. UC—untreated control, C28—exposure to 4°C for 28 consecutive days, IMO1—exposure to immobilization on 1 day for 2 h, C28IMO1—exposure to cold for 28 consecutive days with subsequent exposure to a single immobilization for 2 h. Values are mean ± SEM; n, 6–8 rats per group. One-way ANOVA: p = 3.243 × 10− 5, F = 22.4902. *p < 0.05 vs. UC.
