Figures & data
Table I. PCR Primers for genotyping.
Table II. Body weight and food intakes.
Figure 1. Change in body weight from the morning before the first restraint (day 0). Data are mean ± SEM for groups of 9 to 22 mice. The WT mice represent combined groups of WT from CRFR1-KO and CRFR2-KO colonies. Asterisks indicate days on which there was a significant difference in weight change between Control and RRS mice. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; EPM, elevated plus maze exposure; KO, gene deletion; L/D, light/dark choice test; RRS, daily restraint stress for 3 days; WT, wild type.
![Figure 1. Change in body weight from the morning before the first restraint (day 0). Data are mean ± SEM for groups of 9 to 22 mice. The WT mice represent combined groups of WT from CRFR1-KO and CRFR2-KO colonies. Asterisks indicate days on which there was a significant difference in weight change between Control and RRS mice. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; EPM, elevated plus maze exposure; KO, gene deletion; L/D, light/dark choice test; RRS, daily restraint stress for 3 days; WT, wild type.](/cms/asset/5f6b6f02-50a6-479f-9ca5-219d42f2838e/ists_a_420925_f0001_b.gif)
Figure 2. Food intake of the mice represented as total intake for 3 days before, during, and after RRS. The asterisk indicates a significant difference (P < 0.05) in intake (g/mse[mouse]) of Control versus RRS mice. Data are mean ± SEM for groups of 9–22 mice. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; KO, gene deletion; RRS, daily restraint stress for 3 days; WT, wild type.
![Figure 2. Food intake of the mice represented as total intake for 3 days before, during, and after RRS. The asterisk indicates a significant difference (P < 0.05) in intake (g/mse[mouse]) of Control versus RRS mice. Data are mean ± SEM for groups of 9–22 mice. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; KO, gene deletion; RRS, daily restraint stress for 3 days; WT, wild type.](/cms/asset/21466aef-bdb4-4085-a413-b8243e4884b0/ists_a_420925_f0002_b.gif)
Figure 3. Behavior measured during 5 min in the elevated plus maze (EPM) on day 11 of the experimental period, 9 days after the end of daily RRS for 3 days (RRS). Values on any specific axis that do not share a common superscript are significantly different at P < 0.05. Data are mean ± SEM for groups of 9–22 mice. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; KO, gene deletion; WT, wild type.
![Figure 3. Behavior measured during 5 min in the elevated plus maze (EPM) on day 11 of the experimental period, 9 days after the end of daily RRS for 3 days (RRS). Values on any specific axis that do not share a common superscript are significantly different at P < 0.05. Data are mean ± SEM for groups of 9–22 mice. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; KO, gene deletion; WT, wild type.](/cms/asset/d05a8b40-b4cb-4f66-b820-a6137ed9a01a/ists_a_420925_f0003_b.gif)
Figure 4. Behavior measured in the light/ dark (L/D) choice test on day 14 of the experimental period, 12 days after the end of daily restraint for 3 days (RRS). Values on an axis that do not share a common superscript are significantly different at P < 0.05. Data are mean ± SEM for groups of 9–22 mice. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; KO, gene deletion; WT, wild type.
![Figure 4. Behavior measured in the light/ dark (L/D) choice test on day 14 of the experimental period, 12 days after the end of daily restraint for 3 days (RRS). Values on an axis that do not share a common superscript are significantly different at P < 0.05. Data are mean ± SEM for groups of 9–22 mice. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; KO, gene deletion; WT, wild type.](/cms/asset/c13f1266-096e-45e4-b1e2-a1c1853394e6/ists_a_420925_f0004_b.gif)
Figure 5. Serum corticosterone measured before (time 0) and 25 min (time 25) after the start of testing in the EPM on day 11 of the experimental period. Values that do not share a common superscript are significantly different at P < 0.05. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; CTRL, control; KO, gene deletion; RRS, daily restraint stress for 3 days; WT, wild type.
![Figure 5. Serum corticosterone measured before (time 0) and 25 min (time 25) after the start of testing in the EPM on day 11 of the experimental period. Values that do not share a common superscript are significantly different at P < 0.05. ADX, adrenalectomised and corticosterone replacement; CRFR, corticotropin releasing factor receptor; CTRL, control; KO, gene deletion; RRS, daily restraint stress for 3 days; WT, wild type.](/cms/asset/01da81c5-bd10-4a68-9fb8-ea67d323b2dd/ists_a_420925_f0005_b.gif)