Microencapsulation of sodium alendronate reduces drug mucosal damage in rats

Figures & data

Table 1.  Physico-chemical characteristics of microparticles.

Sample	EE^** (%)	Drug loading (mg/g)	Particle size distribution
			d0.1 (μm)	d0.5 (μm)	d0.9 (μm)	d4.3 (μm)	span
MP-EUD	100.1 ± 0.8	181.9 ± 2.5	4.0	12.9	25.3	14.1	1.7
MP-EUDp	—	—	3.0	15.2	32.8	17.1	1.9
MPE-E4M	80.7 ± 2.5	153.7 ± 1.9	1.8	6.4	13.7	7.3	1.8
MPE-E4Mp	—	—	2.6	8.8	18.9	10.4	1.8

* MP-EUDp and MPE-E4Mp = unloaded microparticles. ** Encapsulation efficiency.

Figure 1.  Photomicrographs of MP-EUD (A) and MPE-E4M (B) formulations (bar = 10 μm).

Figure 2.  Dissolution profiles of MP-EUD and MPE-E4M formulations in 0.1 M HCl pH 1.2.

Figure 3.  Dissolution profiles of pure sodium alendronate, MP-EUD and MPE-E4M formulations in phosphate buffer solution pH 6.8.

Table 2.  Parameters calculated by monoexponential, biexponential and power law models for MP-EUD and MPE-E4M.

	MP-EUD	MPE-E4M
Monoexponential
k (min^−1)	0.0161 ± 0.0012	0.0047 ± 0.0008
r^2	0.9859	0.9767
MSC	3.8347	3.1440
Biexponential
α (min^−1)	0.0155 ± 0.0009	0.3904 ± 0.0011
β (min^−1)	0.0149 ± 0.0015	0.0040 ± 0.0012
a	0.3643 ± 0.0008	0.1186 ± 0.0008
b	0.6178 ± 0.0018	0.8815 ± 0.0010
r^2	0.9618	0.9882
MSC	2.6445	3.8680
Korsmeyer-Peppas
a	0.050 ± 0.008	0.043 ± 0.008
n	0.590 ± 0.006	0.489 ± 0.011
r^2	0.9876	0.9972

Table 3.  Effects of the treatments on ulcerative lesion index, total area of lesion, and percentage of lesion area.

Treatment (p.o.)	Total area of lesion (mm^2)	% of lesion area	Ulcerative Lesion Index
Control (water)	not found	—	—
MP-EUD	not found	—	—
MP-EUDp	not found	—	—
MPE-E4M	not found	—	—
MPE-E4Mp	not found	—	—
Sodium alendronate solution	3.99 ± 0.90	0.53 ± 0.12	6.50 ± 0.61

Results are mean ± SEM for six female rats.