Figures & data
Table 1. The physicochemical characterization of PTX-loaded polymeric micelles (n = 3).
Figure 1. XRD spectra of (a) PTX, (b) TS-CS-PEG-FA, (c) physical mixture of PTX and TS-CS-PEG-FA and (d) PTX-loaded TS-CS-PEG-FA.
![Figure 1. XRD spectra of (a) PTX, (b) TS-CS-PEG-FA, (c) physical mixture of PTX and TS-CS-PEG-FA and (d) PTX-loaded TS-CS-PEG-FA.](/cms/asset/f439604f-58a2-43dd-80cb-3ce0f3a3c24f/idrd_a_954281_f0001_b.jpg)
Figure 2. DSC spectra of (a) PTX, (b) TS-CS-PEG-FA, (c) physical mixture of PTX and TS-CS-PEG-FA and (d) PTX-loaded TS-CS-PEG-FA.
![Figure 2. DSC spectra of (a) PTX, (b) TS-CS-PEG-FA, (c) physical mixture of PTX and TS-CS-PEG-FA and (d) PTX-loaded TS-CS-PEG-FA.](/cms/asset/caf33147-3c3f-4b4a-a399-e7c4f011d66c/idrd_a_954281_f0002_b.jpg)
Figure 3. Fluorescence image after the cells were incubated with fluorescent (a) TS-CS micelles and (b) TSCS.
![Figure 3. Fluorescence image after the cells were incubated with fluorescent (a) TS-CS micelles and (b) TSCS.](/cms/asset/0430eb98-65d8-4b6f-bad9-314ad8be66a1/idrd_a_954281_f0003_c.jpg)
Figure 4. The cellular uptake percentage of FITC/CS-TS and FITC/TS-CS-PEG-FA micelles in 4T1 cells (the polymer concentrations were 20 μg/ml).
![Figure 4. The cellular uptake percentage of FITC/CS-TS and FITC/TS-CS-PEG-FA micelles in 4T1 cells (the polymer concentrations were 20 μg/ml).](/cms/asset/10fbad81-5bff-491f-bfd3-6a6fcf7231e5/idrd_a_954281_f0004_b.jpg)
Figure 5. In vitro cytotoxicity of various PTX formulations and blank micelles against 4T1 cell line after 72 h incubation. Data were plotted as the mean ± SD of three measurements.
![Figure 5. In vitro cytotoxicity of various PTX formulations and blank micelles against 4T1 cell line after 72 h incubation. Data were plotted as the mean ± SD of three measurements.](/cms/asset/219cdf64-feb8-483b-b22c-d5aff1428a32/idrd_a_954281_f0005_b.jpg)
Figure 6. Effect of free folic acid on viability of 4T1 cells incubated with PTX/TS-CS-PEG-FA micelles at PTX concentration of 1.17 μM.
![Figure 6. Effect of free folic acid on viability of 4T1 cells incubated with PTX/TS-CS-PEG-FA micelles at PTX concentration of 1.17 μM.](/cms/asset/8503c5a8-173c-4c09-9fa7-ec5a2139fdad/idrd_a_954281_f0006_b.jpg)
Figure 7. Tumor volume change in mice with PTX/TS-CS, PTX/TS-CS-PEG-FA, Anzatax®, blank micelles or saline (n = 6) (a) images of whole mice were taken at 25 days after tumor inoculation (b).
![Figure 7. Tumor volume change in mice with PTX/TS-CS, PTX/TS-CS-PEG-FA, Anzatax®, blank micelles or saline (n = 6) (a) images of whole mice were taken at 25 days after tumor inoculation (b).](/cms/asset/5cfafd1d-e992-4b58-a52d-0adc0e7d3ba8/idrd_a_954281_f0007_c.jpg)
Figure 8. H&E-stained tumor slices from mice on the 25nd day after treatment with (a) saline, (b) Anzatax®, (c) PTX/TS-CS and (d) PTX/TS-CS-PEG-FA. Images were obtained under Olympus IX71 optical microscope.
![Figure 8. H&E-stained tumor slices from mice on the 25nd day after treatment with (a) saline, (b) Anzatax®, (c) PTX/TS-CS and (d) PTX/TS-CS-PEG-FA. Images were obtained under Olympus IX71 optical microscope.](/cms/asset/191087e5-7734-4307-b3f5-1aee65c06b19/idrd_a_954281_f0008_c.jpg)
Table 2. In vivo anti-tumor effect of PTX formulations against 4T1 tumor in Balb/c mice (n = 6).
Figure 9. Mean concentration–time profile of PTX in Balb/c mice plasma after i.v. administration of Anzatax® and PTX/TS-CS-PEG-FA. Each point represents the mean ± SD (n = 3).
![Figure 9. Mean concentration–time profile of PTX in Balb/c mice plasma after i.v. administration of Anzatax® and PTX/TS-CS-PEG-FA. Each point represents the mean ± SD (n = 3).](/cms/asset/9ae0c782-4605-4b4b-8bac-ccf2296466a5/idrd_a_954281_f0009_b.jpg)
Figure 10. The PTX concentration in mice receiving Anzatax® or PTX/TS-CS-PEG-FA in tissues at different time points (0.083, 0.5, 1, 2, 4, 8 and 12 h) (mean ± SD, n = 5).
![Figure 10. The PTX concentration in mice receiving Anzatax® or PTX/TS-CS-PEG-FA in tissues at different time points (0.083, 0.5, 1, 2, 4, 8 and 12 h) (mean ± SD, n = 5).](/cms/asset/97f610f5-06a4-47f9-b370-c1f97a6ec163/idrd_a_954281_f0010_b.jpg)