Preparation and evaluation of nanoparticles loaded ophthalmic in situ gel

Figures & data

Table 1. Formulation table of in situ gel.

Ingredients	Quantity
Gellan gum	200 mg
Deionized water	50 ml
Benzalkonium chloride	0.2%

Table 2. Formulation table for nanoparticles.

Ingredients	F1	F2
Moxifloxacin HCl (mg)	250	250
Poloxamer 407 (mg)	500	500
Eudragit (mg)	400	400
PVA (mg)	–	500
Acetone (ml)	10	10
Water (ml)	50	50

Figure 1. FTIR spectra of moxifloxacin.

Figure 2. FTIR spectra of moxifloxacin + PVA + Eudrgit RL100 + Poloxamer 407.

Figure 3. FTIR spectra of moxifloxacin + Poloxamer 407 + Eudragit RL100.

Figure 4. Overlay FTIR spectra of moxifloxacin (PVA + Eudrgit RL100 + Poloxamer 407, Poloxamer 407 + Eudragit RL100).

Table 3. Particle size, PDI, zeta potential, % EE, clarity and pH.

Formulation	Particle size	PDI	Zeta potential	Entrapment efficiency (%)	Clarity	pH
F1	101.4	0.43	5.3	79.5	Clear	6.7
	105.8	0.5	6.9	75.23	Clear	6.9
F2	159.6	0.8	6.53	71.25	Clear	6.5
	172.3	0.57	8.89	73.48	Clear	6.7

Table 4. Drug content, gelling capacity and viscocity.

			Viscosity
Formulation	Drug content	Gelling capacity	Before gelation	After gelation
F1	86.019%	+++	123	843
F2	77.833%	+++	128	856

Table 5. Antimicrobial effect.

Organism	Standard ZOI	Test ZOI	Percent efficacy
Staphylococcus aureus	1.5	1.4	93.33
Bacillus subtilis	1.1	1.0	90.90
Escherichia Coli	1.6	1.5	96.15
Candida albicans	1.9	1.8	96.55

Table 6. Sterility test.

		Parameters
Organism	+ve Control	−ve Control	F1	F2
Bacteria	Turbid	Clear	Clear	Clear
Fungi	Turbid	Clear	Clear	Clear

Table 7. Effect of sterilization.

	Drug content		pH		Viscosity		Gelling capacity
Formulation	Before	After	Before	After	Before	After	Before	After
F1	86.019	85.64	6.7	6.6	123	121	+++	+++
F2	77.833	77.447	6.5	6.5	128	125	+++	+++

Figure 5. Comparison of release profile of optimized batch.

Figure 6. Cornea section of (a) healthy eye; (b) formulation treated eye.

Figure 7. Image of confocal microscopy.