In vitro and in vivo study of Baicalin-loaded mixed micelles for oral delivery

Figures & data

Figure 1. XRD spectra of BC, ST-P123-MMs, BC+ST-P123 physical mixture, and BC-loaded ST-P123-MMs.

Figure 2. Cumulative released amount of DTX as a function of time in SGF (0–2 h, pH 1.2) and in SIF (2–48 h, pH 6.8) (n = 3).

Figure 3. The absorption curves of BC solution (a) and BC-loaded ST-P123-MMs (b) in the rat intestine in situ for 6 h with various concentrations. Each data represents the mean ± standard deviation of three rats.

Table 1. Intestinal absorption kinetic parameters of BC solution and BC-loaded ST-P123-MMs in situ at different concentrations (results represent mean values ± SD, n = 3).

Drug concentration (μg/mL)	Ka ± SD (h^−1)	t1/2 (h)	P ± SD (%)
BC solution
27	0.0270 ± 0.0093	25.6667	15.57 ± 4.43
54	0.0218 ± 0.0035	31.7890	12.83 ± 1.35
108	0.0254 ± 0.0069	27.2835	14.96 ± 5.08
BC-loaded ST-P123-MMs
27	0.0789 ± 0.0041	8.7833	42.69 ± 1.10
54	0.0998 ± 0.2860	6.9474	50.04 ± 9.47
108	0.0528 ± 0.0091	13.1374	28.37 ± 6.60

Figure 4. Fluorescence microscopy (FLM) images of Caco-2 cells after 2 h incubation with coumarin-6 solution (a) and coumarin-6-loaded ST-P123-MMs (b).

Figure 5. Mean plasma concentration–time curves of BC suspension and BC-loaded ST-P123-MMs after oral administration (n = 3).

Table 2. Pharmacokinetic parameters of BC suspension and BC-loaded ST-P123-MMs via oral administration (results represent mean values ± SD, n = 3).

Pharmacokinetic parameters	BC suspension	BC-loaded ST-P123-MMs
Dose (mg/kg)	200	200
Tmax (h)	4.83 ± 3.88	8 ± 0.00
Cmax (μg/mL)	7.73 ± 1.40	13.68 ± 1.68
AUC (h μg/mL)	266.19 ± 17.04	409.76 ± 20.71
MRT (h)	21.84 ± 0.59	21.96 ± 0.66